GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19592575

From GONUTS
Jump to: navigation, search
Citation

Zanet, J, Stramer, B, Millard, T, Martin, P, Payre, F and Plaza, S (2009) Fascin is required for blood cell migration during Drosophila embryogenesis. Development 136:2557-65

Abstract

Fascin is well characterized in vitro as an actin-bundling protein and its increased expression is correlated with the invasiveness of various cancers. However, the actual roles and regulation of Fascin in vivo remain elusive. Here we show that Fascin is required for the invasive-like migration of blood cells in Drosophila embryos. Fascin expression is highly regulated during embryonic development and, within the blood lineage, is specific to the motile subpopulation of cells, which comprises macrophage-like plasmatocytes. We show that Fascin is required for plasmatocyte migration, both as these cells undergo developmental dispersal and during an inflammatory response to epithelial wounding. Live analyses further demonstrate that Fascin localizes to, and is essential for the assembly of, dynamic actin-rich microspikes within plasmatocyte lamellae that polarize towards the direction of migration. We show that a regulatory serine of Fascin identified from in vitro studies is not required for in vivo cell motility, but is crucial for the formation of actin bundles within epithelial bristles. Together, these results offer a first glimpse into the mechanisms regulating Fascin function during normal development, which might be relevant for understanding the impact of Fascin in cancers.

Links

PubMed Online version:10.1242/dev.036517

Keywords

Amino Acid Sequence; Animal Structures/embryology; Animal Structures/ultrastructure; Animals; Blood Cells/cytology; Blood Cells/metabolism; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Movement; Cell Polarity; Cell Survival; Cytoskeleton/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/ultrastructure; Embryonic Development/genetics; Gene Expression Regulation, Developmental; Inflammation/metabolism; Inflammation/pathology; Microfilament Proteins/chemistry; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Molecular Sequence Data; Morphogenesis; Mutation/genetics; Oogenesis; Organ Specificity; Phosphorylation; Pseudopodia/metabolism; Serine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:SING

located_in

GO:0015629: actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:SING

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:SING

involved_in

GO:0035099: hemocyte migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:SING

part_of

GO:0015629: actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:SING

involved_in

GO:0007163: establishment or maintenance of cell polarity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:SING

involved_in

GO:0042060: wound healing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.