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PMID:19567473

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Citation

Campbell, K, Knust, E and Skaer, H (2009) Crumbs stabilises epithelial polarity during tissue remodelling. J. Cell. Sci. 122:2604-12

Abstract

The apicobasal polarity of epithelia depends on the integrated activity of apical and basolateral proteins, and is essential for tissue integrity and body homeostasis. Yet these tissues are frequently on the move as they are sculpted by active morphogenetic cell rearrangements. How does cell polarity survive these stresses? We analyse this question in the renal tubules of Drosophila, a tissue that undergoes dramatic morphogenetic change as it develops. Here we show that, whereas the Bazooka and Scribble protein groups are required for the establishment of tubule cell polarity, the key apical determinant, Crumbs, is required for cell polarity in the tubules only from the time when morphogenetic movements start. Strikingly, if these movements are stalled, polarity persists in the absence of Crumbs. Similar rescue of the ectodermal phenotype of the crumbs mutant when germ-band extension is reduced suggests that Crumbs has a specific, conserved function in stabilising cell polarity during tissue remodelling rather than in its initial stabilisation. We also identify a requirement for the exocyst component Exo84 during tissue morphogenesis, which suggests that Crumbs-dependent stability of epithelial polarity is correlated with a requirement for membrane recycling and targeted vesicle delivery.

Links

PubMed PMC2909315 Online version:10.1242/jcs.047183

Keywords

Adherens Junctions/metabolism; Animals; Cell Polarity/physiology; Cells, Cultured; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila Proteins/physiology; Drosophila melanogaster/embryology; Embryo, Nonmammalian/physiology; Epithelial Cells/physiology; Female; Fluorescent Antibody Technique, Indirect; Gene Expression Regulation, Developmental; Guanylate Kinase/genetics; Guanylate Kinase/metabolism; Immunoenzyme Techniques; Intracellular Signaling Peptides and Proteins/physiology; Kidney Tubules/embryology; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Proteins/physiology; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Morphogenesis/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:O96782

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CRB

involved_in

GO:0007443: Malpighian tubule morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CRB

involved_in

GO:0061336: cell morphogenesis involved in Malpighian tubule morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CRB

involved_in

GO:0045218: zonula adherens maintenance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CRB

involved_in

GO:0035090: maintenance of apical/basal cell polarity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q0KHR3

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:SCRIB

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q7KRZ3

involved_in

GO:0007163: establishment or maintenance of cell polarity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q86NX9

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SWU0

involved_in

GO:0007163: establishment or maintenance of cell polarity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VBI4

involved_in

GO:0007163: establishment or maintenance of cell polarity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VX75

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:X2JCB3

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:X2JFU8

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:LAP4

involved_in

GO:0072002: Malpighian tubule development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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