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PMID:19560455

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Citation

Baker, MA, Hetherington, L, Curry, B and Aitken, RJ (2009) Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa; its implications during capacitation. Dev. Biol. 333:57-66

Abstract

Upon ejaculation, spermatozoa undergo a series of post-translational modifications in a process known as capacitation in order to prepare for fertilization. In the absence of capacitation, fertilization cannot occur. Spermatozoa are unusual in that one of the hallmarks of capacitation is a global up-regulation in phosphotyrosine expression, which is known to be mediated upstream by PKA. Little is known about the signaling events downstream of PKA apart from the involvement of SRC, as a key mediator of PKA-induced tyrosine phosphorylation in the sperm tail. Here we describe the presence of c-Abl in mouse spermatozoa. In vitro analysis confirmed that PKA can up-regulate c-Abl kinase activity. In vivo, this tyrosine kinase was found to associate, and become threonine phosphorylated by PKA in the sperm flagellum. By treating spermatozoa with hemolysin we could demonstrate that a significant proportion of the tyrosine phosphorylation associated with capacitation could be suppressed by the c-Abl inhibitor, Gleevac. This is the first report of c-Abl being up-regulated by PKA for any cell type. We present a model, whereby these kinases may operate together with SRC to ensure optimal levels of tyrosine phosphorylation in the sperm flagellum during the attainment of a capacitated state.

Links

PubMed Online version:10.1016/j.ydbio.2009.06.022

Keywords

Animals; Cyclic AMP-Dependent Protein Kinases/metabolism; Enzyme Activation; Flagella/physiology; Male; Mice; Phosphorylation; Piperazines/pharmacology; Proto-Oncogene Proteins c-abl/antagonists & inhibitors; Proto-Oncogene Proteins c-abl/metabolism; Pyrimidines/pharmacology; Sperm Capacitation/drug effects; Sperm Capacitation/physiology; Spermatozoa/physiology; Threonine/metabolism; Tyrosine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:KAPCA

involved_in

GO:0048240: sperm capacitation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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