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PMID:19548979

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Citation

Guillaumot, D, Guillon, S, Déplanque, T, Vanhee, C, Gumy, C, Masquelier, D, Morsomme, P and Batoko, H (2009) The Arabidopsis TSPO-related protein is a stress and abscisic acid-regulated, endoplasmic reticulum-Golgi-localized membrane protein. Plant J. 60:242-56

Abstract

The Arabidopsis gene At2g47770 encodes a membrane-bound protein designated AtTSPO (Arabidopsis thaliana TSPO-related). AtTSPO is related to the bacterial outer membrane tryptophan-rich sensory protein (TspO) and the mammalian mitochondrial 18-kDa translocator protein (18 kDa TSPO), members of the group of TspO/MBR domain-containing membrane proteins. In this study we show that AtTSPO is mainly detected in dry seeds, but can be induced in vegetative tissues by osmotic or salt stress or abscisic acid (ABA) treatment, corroborating available transcriptome data. Using subcellular fractionation, immunocytochemistry and fluorescent protein tagging approaches we present evidence that AtTSPO is targeted to the secretory pathway in plants. Induced or constitutively expressed AtTSPO can be detected in the endoplasmic reticulum and the Golgi stacks of plant cells. AtTSPO tagged with fluorescent protein in transgenic plants (Arabidopsis and tobacco) was mainly detected in the Golgi stacks of leaf epidermal cells. Constitutive expression of AtTSPO resulted in increased sensitivity to NaCl, but not to osmotic stress, and in reduced greening of cultured Arabidopsis cells under light growing conditions. Transgenic Arabidopsis plants overexpressing AtTSPO were more sensitive to ABA-induced growth inhibition, indicating that constitutive expression of AtTSPO may enhance ABA sensitivity. AtTSPO is rapidly downregulated during seed imbibition, and the ABA-dependent induction in plant is transient. Downregulation of AtTSPO seems to be boosted by treatment with aminolevulinic acid. Taken together, these results suggest that AtTSPO is a highly regulated protein, induced by abiotic stress to modulate, at least in part, transient intracellular ABA-dependent stress perception and/or signalling.

Links

PubMed Online version:10.1111/j.1365-313X.2009.03950.x

Keywords

Abscisic Acid/metabolism; Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Endoplasmic Reticulum/metabolism; Gene Expression Regulation, Plant; Golgi Apparatus/metabolism; Molecular Sequence Data; Osmotic Pressure; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Plants, Genetically Modified/metabolism; Seeds/genetics; Sequence Alignment; Signal Transduction; Sodium Chloride/pharmacology; Stress, Physiological

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TSPO

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSPO

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSPO

acts_upstream_of_or_within

GO:0006970: response to osmotic stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TSPO

located_in

GO:0005795: Golgi stack

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSPO

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TSPO

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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