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PMID:19544087

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Citation

Liu, L, Li, Y, Lin, J, Liang, Q, Sheng, X, Wu, J, Huang, R, Liu, S and Li, Y (2010) Connexin43 interacts with Caveolin-3 in the heart. Mol. Biol. Rep. 37:1685-91

Abstract

Gap junctions (GJs), collections of multiple intercellular channels between neighboring cells, are specialized channels facilitating intercellular electrical and chemical communication. GJs are important for synchronizing coupling and coordinated contraction in the heart, and are crucial regulators of heart gene transcription, cardiac development, and protection of ischemic cardiomyocytes through second messenger communication. Identification of proteins that interact with Connexin43 (Cx43), the predominant protein in cardiac GJs, may contribute to the understanding of GJ functional regulation. Using a yeast two-hybrid system, we identified Caveolin-3 (Cav3) as a new Cx43-interacting protein. This interaction was confirmed by co-immunoprecipitation and co-localization experiments. CX43 interacts with Cav3, suggesting that Cav3 may participate in the functional regulation of GJs.

Links

PubMed Online version:10.1007/s11033-009-9584-5

Keywords

Base Sequence; Caveolin 3/metabolism; Connexin 43/metabolism; Electrophoresis, Polyacrylamide Gel; Green Fluorescent Proteins/metabolism; HeLa Cells; Humans; Immunoprecipitation; Molecular Sequence Data; Myocardium/metabolism; Plasmids/genetics; Protein Binding; Protein Transport; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CAV3

enables

GO:0071253: connexin binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P23242

F

Seeded From UniProt

complete

HUMAN:CAV3

enables

GO:0071253: connexin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:CXA1

enables

GO:0097110: scaffold protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P51637

F

Seeded From UniProt

complete


See also

References

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