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PMID:19543274

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Citation

Rajan, A, Tien, AC, Haueter, CM, Schulze, KL and Bellen, HJ (2009) The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors. Nat. Cell Biol. 11:815-24

Abstract

Cell fate decisions mediated by the Notch signalling pathway require direct cell-cell contact between adjacent cells. In Drosophila melanogaster, an external sensory organ (ESO) develops from a single sensory organ precursor (SOP) and its fate specification is governed by differential Notch activation. Here we show that mutations in actin-related protein-3 (Arp3) compromise Notch signalling, leading to a fate transformation of the ESO. Our data reveal that during ESO fate specification, most endocytosed vesicles containing the ligand Delta traffic to a prominent apical actin-rich structure (ARS) formed in the SOP daughter cells. Using immunohistochemistry and transmission electron microscopy (TEM) analyses, we show that the ARS contains numerous microvilli on the apical surface of SOP progeny. In Arp2/3 and WASp mutants, the surface area of the ARS is substantially reduced and there are significantly fewer microvilli. More importantly, trafficking of Delta-positive vesicles from the basal area to the apical portion of the ARS is severely compromised. Our data indicate that WASp-dependent Arp2/3 actin polymerization is crucial for apical presentation of Delta, providing a mechanistic link between actin polymerization and Notch signalling.

Links

PubMed PMC3132077 Online version:10.1038/ncb1888

Keywords

Actin-Related Protein 2-3 Complex/genetics; Actin-Related Protein 2-3 Complex/metabolism; Actin-Related Protein 2-3 Complex/physiology; Animals; Biological Transport/genetics; Biological Transport/physiology; Blotting, Western; Cell Differentiation/genetics; Cell Differentiation/physiology; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Drosophila melanogaster/ultrastructure; Endocytosis/genetics; Endocytosis/physiology; Gene Expression Regulation, Developmental; Immunohistochemistry; Intracellular Signaling Peptides and Proteins; Membrane Proteins/metabolism; Microscopy, Electron, Transmission; Microscopy, Immunoelectron; Microvilli/metabolism; Microvilli/ultrastructure; Sense Organs/embryology; Sense Organs/metabolism; Sense Organs/ultrastructure; Wiskott-Aldrich Syndrome Protein/genetics; Wiskott-Aldrich Syndrome Protein/metabolism; Wiskott-Aldrich Syndrome Protein/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:O97182

involved_in

GO:0006886: intracellular protein transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ARP3

involved_in

GO:0022416: chaeta development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ARP3

involved_in

GO:0006886: intracellular protein transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q86B74

involved_in

GO:0006886: intracellular protein transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VAT0

involved_in

GO:0006886: intracellular protein transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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