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PMID:19543227

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Citation

Jiang, K, Wang, J, Liu, J, Ward, T, Wordeman, L, Davidson, A, Wang, F and Yao, X (2009) TIP150 interacts with and targets MCAK at the microtubule plus ends. EMBO Rep. 10:857-65

Abstract

The microtubule (MT) cytoskeleton orchestrates the cellular plasticity and dynamics that underlie morphogenesis and cell division. Growing MT plus ends have emerged as dynamic regulatory machineries in which specialized proteins-called plus-end tracking proteins (+TIPs)-bind to and control the plus-end dynamics that are essential for cell division and migration. However, the molecular mechanisms underlying the plus-end regulation by +TIPs at spindle and astral MTs have remained elusive. Here, we show that TIP150 is a new +TIP that binds to end-binding protein 1 (EB1) in vitro and co-localizes with EB1 at the MT plus ends in vivo. Suppression of EB1 eliminates the plus-end localization of TIP150. Interestingly, TIP150 also binds to mitotic centromere-associated kinesin (MCAK), an MT depolymerase that localizes to the plus end of MTs. Suppression of TIP150 diminishes the plus-end localization of MCAK. Importantly, aurora B-mediated phosphorylation disrupts the TIP150-MCAK association in vitro. We reason that TIP150 facilitates the EB1-dependent loading of MCAK onto MT plus ends and orchestrates the dynamics at the plus end of MTs.

Links

PubMed PMC2699393 Online version:10.1038/embor.2009.94

Keywords

Amino Acid Sequence; Cell Line; HeLa Cells; Humans; Immunohistochemistry; Kinesin/metabolism; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Microtubules/metabolism; Molecular Sequence Data; Protein Binding; Sequence Homology, Amino Acid; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CRIGR:KIF2C

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5JR59

F

Seeded From UniProt

complete

CRIGR:KIF2C

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q15691

F

Seeded From UniProt

complete

CRIGR:KIF2C

colocalizes_with

GO:0005881: cytoplasmic microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MARE1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P70096

F

Seeded From UniProt

complete

HUMAN:MARE1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5JR59

F

Seeded From UniProt

complete

HUMAN:MARE1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99661

F

Seeded From UniProt

complete

HUMAN:MTUS2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P70096

F

Seeded From UniProt

complete

HUMAN:MTUS2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q15691

F

Seeded From UniProt

complete

HUMAN:MTUS2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99661

F

Seeded From UniProt

complete

HUMAN:KIF2C

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q15691

F

Seeded From UniProt

complete

HUMAN:KIF2C

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5JR59

F

Seeded From UniProt

complete

HUMAN:MTUS2

enables

GO:0042803: protein homodimerization activity

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5JR59

F

Seeded From UniProt

complete

HUMAN:MTUS2

enables

GO:0008017: microtubule binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MTUS2

colocalizes_with

GO:0005881: cytoplasmic microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MTUS2

colocalizes_with

GO:0005813: centrosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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