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PMID:19531358

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Citation

Massarwa, R, Schejter, ED and Shilo, BZ (2009) Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. Dev. Cell 16:877-88

Abstract

Apical localization of filamentous actin (F-actin) is a common feature of epithelial tubes in multicellular organisms. However, its origins and function are not known. We demonstrate that the Diaphanous (Dia)/Formin actin-nucleating factor is required for generation of apical F-actin in diverse types of epithelial tubes in the Drosophila embryo. Dia itself is apically localized both at the RNA and protein levels, and apical localization of its activators, including Rho1 and two guanine exchange factor proteins (Rho-GEFs), contributes to its activity. In the absence of apical actin polymerization, apical-basal polarity and microtubule organization of tubular epithelial cells remain intact; however, secretion through the apical surface to the lumen of tubular organs is blocked. Apical secretion also requires the Myosin V (MyoV) motor, implying that secretory vesicles are targeted to the apical membrane by MyoV-based transport, along polarized actin filaments nucleated by Dia. This mechanism allows efficient utilization of the entire apical membrane for secretion.

Links

PubMed Online version:10.1016/j.devcel.2009.04.010

Keywords

Actin Cytoskeleton/metabolism; Actins/metabolism; Animals; Biological Transport; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Polarity; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/embryology; Drosophila melanogaster/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Epithelium/secretion; Gene Expression Regulation, Developmental; Guanine Nucleotide Exchange Factors/metabolism; Microfilament Proteins/metabolism; Mutation/genetics; Myosin Type V/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Secretory Vesicles/metabolism; rho GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:DIA

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:DIA

involved_in

GO:0009306: protein secretion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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