GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:19519766
| Citation |
Daran-Lapujade, P, Daran, JM, Luttik, MA, Almering, MJ, Pronk, JT and Kötter, P (2009) An atypical PMR2 locus is responsible for hypersensitivity to sodium and lithium cations in the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D. FEMS Yeast Res. 9:789-92 |
|---|---|
| Abstract |
Saccharomyces cerevisiae strains belonging to the CEN.PK family are widely used in fundamental and applied yeast research. These strains have been reported to be hypersensitive to sodium ions and a previous microarray-based genotyping study indicated an atypical organization of the PMR2 locus. In other S. cerevisiae strains, this locus harbours one to five ENA genes that encode plasma membrane sodium-pumping ATPases. Sequence analysis of the PMR2 locus in S. cerevisiae CEN.PK113-7D revealed the presence of a new ENA gene that showed substantial sequence differences, both at the nucleotide level and at the predicted amino acid sequence level, with previously described ENA genes. The presence of this single and atypical ENA gene correlated with hypersensitivity to sodium and, in particular, to lithium ions. The native ENA6 gene was transcriptionally induced by sodium and lithium ions, but, apparently, the capacity for sodium export upon full induction was insufficient to achieve the levels of sodium and lithium ion tolerance observed in other S. cerevisiae strains. The sodium and lithium hypersensitivity of CEN.PK strains, which is potentially detrimental during cultivation in sodium-rich media, could, however, be suppressed by overexpression of ENA6. |
| Links |
PubMed Online version:10.1111/j.1567-1364.2009.00530.x |
| Keywords |
Antifungal Agents; Cations/pharmacology; DNA, Fungal/genetics; Lithium/pharmacology; Microbial Sensitivity Tests; Saccharomyces cerevisiae/drug effects; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Analysis, DNA; Sodium/pharmacology; Sodium-Potassium-Exchanging ATPase/genetics; Sodium-Potassium-Exchanging ATPase/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
See also
References
See Help:References for how to manage references in GONUTS.
