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PMID:19509291
| Citation |
Tashiro, K, Tsunematsu, T, Okubo, H, Ohta, T, Sano, E, Yamauchi, E, Taniguchi, H and Konishi, H (2009) GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling. J. Biol. Chem. 284:20206-14 |
|---|---|
| Abstract |
Adaptor proteins for the various growth factor receptors play a crucial role in signal transduction through tyrosine phosphorylation. Several candidates for adaptor proteins with potential effects on the epidermal growth factor (EGF) receptor-mediated signaling pathway have been identified by recent phosphoproteomic studies. Here, we focus on a novel protein, GAREM (Grb2-associated and regulator of Erk/MAPK) as a downstream molecule of the EGF receptor. GAREM is phosphorylated at tyrosine 105 and 453 after EGF stimulation. Grb2 was identified as its binding partner, and the proline-rich motifs of GAREM are recognized by the N- and C-terminal SH3 domains of Grb2. In addition, the tyrosine phosphorylations of GAREM are necessary for its binding to Grb2. Because the amino acid sequence surrounding tyrosine 453 is similar to the immunoreceptor tyrosine-based inhibitory motif, Shp2, a positive regulator of Erk, binds to GAREM in this phosphorylation-dependent manner. Consequently, Erk activation in response to EGF stimulation is regulated by the expression of GAREM in COS-7 and HeLa cells, which occurs independent of the presence of other binding proteins, such as Gab1 and SOS, to the activated EGF receptor. Furthermore, the expression of GAREM has an effect on the transformation activity of cultured cells. Together, these findings suggest that GAREM plays a key role in the ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells. |
| Links |
PubMed PMC2740447 Online version:10.1074/jbc.M109.021139 |
| Keywords |
Amino Acid Motifs; Animals; Binding Sites; COS Cells; Cercopithecus aethiops; Extracellular Signal-Regulated MAP Kinases/metabolism; GRB2 Adaptor Protein/analysis; GRB2 Adaptor Protein/genetics; GRB2 Adaptor Protein/metabolism; Gene Expression; HeLa Cells; Humans; Molecular Sequence Data; Phosphorylation; Proline/chemistry; Proline/metabolism; Protein Binding; Protein Tyrosine Phosphatase, Non-Receptor Type 11/metabolism; Receptor, Epidermal Growth Factor/metabolism; Tyrosine/metabolism; src Homology Domains |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q9H706 |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:GRB2 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q9H706 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:GRB2 |
enables |
GO:0017124: SH3 domain binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q9H706 |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:GARE1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q06124 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:GARE1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P62993 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:GARE1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P00533 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:GARE1 |
involved_in |
GO:0007173: epidermal growth factor receptor signaling pathway |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:GARE1 |
located_in |
GO:0005886: plasma membrane |
ECO:0000303: author statement without traceable support used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:GARE1 |
involved_in |
GO:0008284: positive regulation of cell population proliferation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:GARE1 |
enables |
GO:0070064: proline-rich region binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:GARE1 |
involved_in |
GO:0070374: positive regulation of ERK1 and ERK2 cascade |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:GARE1 |
involved_in |
GO:0071364: cellular response to epidermal growth factor stimulus |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
See also
References
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