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PMID:19506555

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Citation

Mravec, J, Skůpa, P, Bailly, A, Hoyerová, K, Krecek, P, Bielach, A, Petrásek, J, Zhang, J, Gaykova, V, Stierhof, YD, Dobrev, PI, Schwarzerová, K, Rolcík, J, Seifertová, D, Luschnig, C, Benková, E, Zazímalová, E, Geisler, M and Friml, J (2009) Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter. Nature 459:1136-40

Abstract

The plant signalling molecule auxin provides positional information in a variety of developmental processes by means of its differential distribution (gradients) within plant tissues. Thus, cellular auxin levels often determine the developmental output of auxin signalling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell. In particular, PIN auxin-efflux-carrier-mediated, directional transport between cells is crucial for generating auxin gradients. Here we show that Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated development. PIN5 does not have a direct role in cell-to-cell transport but regulates intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmic reticulum (ER), presumably mediating auxin flow from the cytosol to the lumen of the ER. The ER localization of other PIN5-like transporters (including the moss PIN) indicates that the diversification of PIN protein functions in mediating auxin homeostasis at the ER, and cell-to-cell auxin transport at the plasma membrane, represent an ancient event during the evolution of land plants.

Links

PubMed Online version:10.1038/nature08066

Keywords

Arabidopsis/classification; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cells, Cultured; Endoplasmic Reticulum/metabolism; Gene Knockout Techniques; Homeostasis/physiology; Indoleacetic Acids/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutation; Phenotype; Phylogeny; Plant Growth Regulators/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PIN5

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PIN5

acts_upstream_of_or_within

GO:0010252: auxin homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PIN5

acts_upstream_of_or_within

GO:0080162: intracellular auxin transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PIN5

enables

GO:0080161: auxin transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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