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PMID:19481538

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Citation

Yamauchi, H, Miyakawa, N, Miyake, A and Itoh, N (2009) Fgf4 is required for left-right patterning of visceral organs in zebrafish. Dev. Biol. 332:177-85

Abstract

Fgf signaling plays essential roles in many developmental events. To investigate the roles of Fgf4 signaling in zebrafish development, we generated Fgf4 knockdown embryos by injection with Fgf4 antisense morpholino oligonucleotides. Randomized LR patterning of visceral organs including the liver, pancreas, and heart was observed in the knockdown embryos. Prominent expression of Fgf4 was observed in the posterior notochord and Kupffer's vesicle region in the early stages of segmentation. Lefty1, lefty2, southpaw, and pitx2 are known to play crucial roles in LR patterning of visceral organs. Fgf4 was essential for the expression of lefty1, which is necessary for the asymmetric expression of southpaw and pitx2 in the lateral plate mesoderm, in the posterior notochord, and the expression of lefty2 and lefty1 in the left cardiac field. Fgf8 is also known to be crucial for the formation of Kupffer's vesicle, which is needed for the LR patterning of visceral organs. In contrast, Fgf4 was required for the formation of cilia in Kupffer's vesicle, indicating that the role of Fgf4 in the LR patterning is quite distinct from that of Fgf8. The present findings indicate that Fgf4 plays a unique role in the LR patterning of visceral organs in zebrafish.

Links

PubMed Online version:10.1016/j.ydbio.2009.05.568

Keywords

Animals; Body Patterning; Cilia/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Fibroblast Growth Factors/metabolism; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Heart/embryology; Left-Right Determination Factors; Liver/embryology; Liver/metabolism; Mesoderm/metabolism; Notochord/metabolism; Pancreas/embryology; Pancreas/metabolism; Viscera/embryology; Viscera/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:FGF8A

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050714-1,ZFIN:ZDB-MRPHLNO-100511-1

P

Seeded From UniProt

complete

DANRE:Q9DFC9

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100511-1

P

Seeded From UniProt

complete

DANRE:Q9DFC9

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-060831-4

P

Seeded From UniProt

complete

DANRE:Q9DFC9

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050714-1,ZFIN:ZDB-MRPHLNO-100511-1

P

Seeded From UniProt

complete

DANRE:Q9DFC9

involved_in

GO:0070121: Kupffer's vesicle development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100511-1

P

Seeded From UniProt

complete

DANRE:Q9DFC9

involved_in

GO:0060271: cilium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100511-1

P

Seeded From UniProt

complete


See also

References

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