PMID:19464282

Citation	Lee, L, Davies, SE and Liu, JL (2009) The spinal muscular atrophy protein SMN affects Drosophila germline nuclear organization through the U body-P body pathway. Dev. Biol. 332:142-55
Abstract	Survival motor neuron protein (SMN) is the determining factor for the human neurodegenerative disease spinal muscular atrophy (SMA). SMN is critical for small nuclear ribonucleoprotein (snRNP) assembly. Using Drosophila oogenesis as a model system, we show that mutations in smn cause abnormal nuclear organization in nurse cells and oocytes. Germline and mitotic clonal analysis reveals that both nurse cells and oocytes require SMN to maintain normal organization of nuclear compartments including chromosomes, nucleoli, Cajal bodies and histone locus bodies. We previously found that SMN-containing U bodies invariably associate with P bodies (Liu, J. L., and Gall, J. G. (2007). U bodies are cytoplasmic structures that contain uridine-rich small nuclear ribonucleoproteins and associate with P bodies. Proc. Natl. Acad. Sci. U. S. A. 104, 11655-11659.). Multiple lines of evidence implicate SMN in the regulation of germline nuclear organization through the connection of U bodies and P bodies. Firstly, smn germline clones phenocopy mutations for two P body components, Cup and Ovarian tumour (Otu). Secondly, P body mutations disrupt SMN distribution and the organization of U bodies. Finally, mutations in smn disrupt the function and organization of U bodies and P bodies. Taken together, our results suggest that SMN is required for the functional integrity of the U body-P body pathway, which in turn is important for maintaining proper nuclear architecture.
Links	PubMed Online version:10.1016/j.ydbio.2009.05.553
Keywords	Animals; Cell Nucleolus/metabolism; Cell Nucleus/metabolism; Clone Cells; Coiled Bodies/metabolism; Cytoplasmic Structures/metabolism; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/metabolism; Germ Cells/cytology; Germ Cells/metabolism; Heterochromatin/metabolism; Histones/metabolism; Mitosis; Models, Biological; Muscular Atrophy, Spinal/metabolism; Mutation/genetics; Oocytes/cytology; Oocytes/metabolism; Protein Transport; RNA, Small Nuclear/metabolism; RNA-Binding Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:SMN	part_of	GO:0071254: cytoplasmic U snRNP body	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(CL:0000026)|part_of:(CL:0000023)	Seeded From UniProt	complete
DROME:SMN	located_in	GO:0005737: cytoplasm	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(CL:0000477)|part_of:(CL:0000022)|part_of:(CL:0000722)	Seeded From UniProt	complete
DROME:SMN	involved_in	GO:0033962: P-body assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:SMN	located_in	GO:0097504: Gemini of coiled bodies	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(CL:0000026)	Seeded From UniProt	complete
DROME:SMN	involved_in	GO:0051276: chromosome organization	ECO:0000315: mutant phenotype evidence used in manual assertion		P	occurs_in:(CL:0000026)|occurs_in:(CL:0000023)|occurs_in:(CL:0000477)	Seeded From UniProt	complete
DROME:SMN	involved_in	GO:1990194: cytoplasmic U snRNP body assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9VV74	involved_in	GO:1990194: cytoplasmic U snRNP body assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9VV74	part_of	GO:0005737: cytoplasm	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(CL:0000477)|part_of:(CL:0000022)|part_of:(CL:0000722)	Seeded From UniProt	complete
DROME:Q9VV74	part_of	GO:0071254: cytoplasmic U snRNP body	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(CL:0000026)|part_of:(CL:0000023)	Seeded From UniProt	complete
DROME:Q9VV74	involved_in	GO:0051276: chromosome organization	ECO:0000315: mutant phenotype evidence used in manual assertion		P	occurs_in:(CL:0000026)|occurs_in:(CL:0000023)|occurs_in:(CL:0000477)	Seeded From UniProt	complete
DROME:Q9VV74	involved_in	GO:0033962: cytoplasmic mRNA processing body assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9VV74	part_of	GO:0097504: Gemini of coiled bodies	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(CL:0000026)	Seeded From UniProt	complete

References

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