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Lawrence, KA, Jewett, MW, Rosa, PA and Gherardini, FC (2009) Borrelia burgdorferi bb0426 encodes a 2'-deoxyribosyltransferase that plays a central role in purine salvage. Mol. Microbiol. 72:1517-29
Borrelia burgdorferi is an obligate parasite with a limited genome that severely narrows its metabolic and biosynthetic capabilities. Thus survival of this spirochaete in an arthropod vector and mammalian host requires that it can scavenge amino acids, fatty acids and nucleosides from a blood meal or various host tissues. Additionally, the utilization of ribonucleotides for DNA synthesis is further complicated by the lack of a ribonucleotide reductase for the conversion of nucleoside-5'-diphosphates to deoxynucleosides-5'-diphosphates. The data presented here demonstrate that B. burgdorferi must rely on host-derived sources of purine bases, deoxypurines and deoxypyrimidines for the synthesis of DNA. However, if deoxyguanosine (dGuo) is limited in host tissue, the enzymatic activities of a 2'-deoxyribosyltransferase (DRTase, encoded by bb0426), IMP dehydrogenase (GuaB) and GMP synthase (GuaA) catalyse the multistep conversion of hypoxanthine (Hyp) to dGMP for DNA synthesis. This pathway provides additional biochemical flexibility for B. burgdorferi when it colonizes and infects different host tissues.
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Borrelia burgdorferi/enzymology; Borrelia burgdorferi/genetics; Carbon-Nitrogen Ligases/genetics; Carbon-Nitrogen Ligases/metabolism; Cloning, Molecular; DNA, Bacterial/metabolism; Genetic Complementation Test; Hypoxanthine/metabolism; IMP Dehydrogenase/genetics; IMP Dehydrogenase/metabolism; Molecular Sequence Data; Pentosyltransferases/genetics; Pentosyltransferases/metabolism; Purines/metabolism; Sequence Alignment; Sequence Analysis, DNA
|Gene product||Qualifier||GO Term||Evidence Code||with/from||Aspect||Extension||Notes||Status|
|GO:0050144: nucleoside deoxyribosyltransferase activity||
Figure 3B and Table 1 show the ability of the purified protein to transfer deoxyribosyl from one purine base to another.
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