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PMID:19433447

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Citation

Parnell, KM and Bass, BL (2009) Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation. Mol. Cell. Biol. 29:4014-23

Abstract

The biogenesis of the large (60S) ribosomal subunit in eukaryotes involves nucleolar, nucleoplasmic, and cytoplasmic steps. The cytoplasmic protein Rei1, found in all eukaryotes, was previously shown to be necessary for the nuclear reimport of 60S subunit export factor Arx1. In this study we investigate the function of Reh1, a protein with high sequence similarity to Rei1. We demonstrate an overlapping function for Reh1 and Rei1 in the cytoplasmic maturation of the 60S subunit that is independent of Arx1 recycling. We observe that strains lacking both Reh1 and Rei1 accumulate salt-labile 60S subunits, suggesting that Reh1/Rei1 is necessary for the cytoplasmic 60S subunit to adopt its mature, stable form.

Links

PubMed PMC2704747 Online version:10.1128/MCB.01582-08

Keywords

Base Sequence; Cytoplasm/chemistry; Cytoplasm/genetics; Cytoplasm/metabolism; DNA Primers/genetics; DNA, Fungal/genetics; Gene Deletion; Genes, Fungal; Protein Subunits; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Ribosome Subunits, Large, Eukaryotic/chemistry; Ribosome Subunits, Large, Eukaryotic/genetics; Ribosome Subunits, Large, Eukaryotic/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:REH1

involved_in

GO:0042273: ribosomal large subunit biogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000471

P

Seeded From UniProt

complete


See also

References

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