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PMID:19414606

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Citation

Flemming, D, Sarges, P, Stelter, P, Hellwig, A, Böttcher, B and Hurt, E (2009) Two structurally distinct domains of the nucleoporin Nup170 cooperate to tether a subset of nucleoporins to nuclear pores. J. Cell Biol. 185:387-95

Abstract

How individual nucleoporins (Nups) perform their role in nuclear pore structure and function is largely unknown. In this study, we examined the structure of purified Nup170 to obtain clues about its function. We show that Nup170 adopts a crescent moon shape with two structurally distinct and separable domains, a beta-propeller N terminus and an alpha-solenoid C terminus. To address the individual roles of each domain, we expressed these domains separately in yeast. Notably, overexpression of the Nup170 C domain was toxic in nup170Delta cells and caused accumulation of several Nups in cytoplasmic foci. Further experiments indicated that the C-terminal domain anchors Nup170 to nuclear pores, whereas the N-terminal domain functions to recruit or retain a subset of Nups, including Nup159, Nup188, and Pom34, at nuclear pores. We conclude that Nup170 performs its role as a structural adapter between cytoplasmically oriented Nups and the nuclear pore membrane.

Links

PubMed PMC2700402 Online version:10.1083/jcb.200810016

Keywords

Cytoplasm/physiology; Escherichia coli/genetics; Galactose/pharmacology; Glucose/pharmacology; Microscopy, Electron; Nuclear Pore/physiology; Nuclear Pore Complex Proteins/chemistry; Nuclear Pore Complex Proteins/genetics; Nuclear Pore Complex Proteins/physiology; Nuclear Pore Complex Proteins/ultrastructure; Promoter Regions, Genetic; Protein Conformation; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/physiology; Saccharomyces cerevisiae Proteins/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:NU170

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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