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PMID:19411624

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Citation

Masson, P, Lundin, D, Söderbom, F and Young, P (2009) Characterization of a REG/PA28 proteasome activator homolog in Dictyostelium discoideum indicates that the ubiquitin- and ATP-independent REGgamma proteasome is an ancient nuclear protease. Eukaryotic Cell 8:844-51

Abstract

The nuclear proteasome activator REGgamma/PA28gamma is an ATP- and ubiquitin-independent activator of the 20S proteasome and has been proposed to degrade and thereby regulate both a key human oncogene, encoding the coactivator SRC-3/AIB1, and the cyclin-dependent kinase inhibitor p21 (Waf/Cip1). We report the identification and characterization of a PA28/REG homolog in Dictyostelium. Association of a recombinant Dictyostelium REG with the purified Dictyostelium 20S proteasome led to the preferential stimulation of the trypsin-like proteasome peptidase activity. Immunolocalization studies demonstrated that the proteasome activator is localized to the nucleus and is present in growing as well as starving Dictyostelium cells. Our results indicate that the Dictyostelium PA28/REG activator can stimulate both the trypsin-like and chymotrypsin-like activities of the 20S proteasome and supports the idea that the REGgamma-20S proteasome represents an early unique nuclear degradation pathway for eukaryotic cells.

Links

PubMed PMC2698304 Online version:10.1128/EC.00165-08

Keywords

Adenosine Triphosphate/metabolism; Amino Acid Sequence; Animals; Dictyostelium/chemistry; Dictyostelium/genetics; Dictyostelium/metabolism; Molecular Sequence Data; Nuclear Proteins/chemistry; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Peptide Hydrolases/chemistry; Peptide Hydrolases/genetics; Peptide Hydrolases/metabolism; Proteasome Endopeptidase Complex/chemistry; Proteasome Endopeptidase Complex/genetics; Proteasome Endopeptidase Complex/metabolism; Protein Transport; Protozoan Proteins/chemistry; Protozoan Proteins/genetics; Protozoan Proteins/metabolism; Sequence Alignment; Ubiquitin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:PSME

involved_in

GO:0030163: protein catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PSME

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:PSME

part_of

GO:0032991: protein-containing complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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