GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19409199

From GONUTS
Jump to: navigation, search
Citation

Bai, Y, Meng, Z, Cui, M, Zhang, X, Chen, F, Xiao, J, Shen, L and Zhang, Y (2009) An Ang1-Tie2-PI3K axis in neural progenitor cells initiates survival responses against oxygen and glucose deprivation. Neuroscience 160:371-81

Abstract

Neural progenitor cells (NPCs) have the potential to survive brain ischemia and participate in neurogenesis after stroke. However, it is not clear how survival responses are initiated in NPCs. Using embryonic mouse NPCs and the in vitro oxygen and glucose deprivation (OGD) model, we found that angiopoietin-1 (Ang1) could prevent NPCs from OGD-induced apoptosis, as evidenced by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and annexin V labeling. Ang1 significantly elevated tunica intima endothelial kinase 2 (Tie2) autophosphorylation level, suggesting the existence of functional Tie2 receptors on NPCs. NPCs under OGD conditions exhibited reduction of Akt phosphorylation, decrease of the Bcl-2/Bax ratio, activation of caspase-3, cleavage of PARP, and downregulation of beta-catenin and nestin. Ang1 reversed the above changes concomitantly with significant rising of survival rates of NPCs under OGD, but all these effects of Ang1 could be blocked by either soluble extracellular domain of Tie2 Fc fusion protein (sTie2Fc) or the phosphoinositide 3-kinase (PI3K) inhibitor 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one (LY294002). Our findings suggest the existence of an Ang1-Tie2-PI3K signaling axis that is essential in initiation of survival responses in NPCs against cerebral ischemia and hypoxia.

Links

PubMed Online version:10.1016/j.neuroscience.2009.01.076

Keywords

Angiopoietin-1/physiology; Animals; Apoptosis Regulatory Proteins/metabolism; Cell Hypoxia/physiology; Cell Survival/physiology; Cells, Cultured; Embryonic Stem Cells/cytology; Embryonic Stem Cells/metabolism; Glucose/deficiency; Humans; In Situ Nick-End Labeling; Mice; Neurogenesis/physiology; Phosphatidylinositol 3-Kinases/metabolism; Receptor Protein-Tyrosine Kinases/metabolism; Receptor, TIE-2/metabolism; Second Messenger Systems/physiology; Signal Transduction/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ANGP1

acts_upstream_of_or_within

GO:0033138: positive regulation of peptidyl-serine phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:ANGP1

acts_upstream_of_or_within

GO:0014068: positive regulation of phosphatidylinositol 3-kinase signaling

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:ANGP1

acts_upstream_of_or_within

GO:0050731: positive regulation of peptidyl-tyrosine phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:ANGP1

acts_upstream_of_or_within

GO:0043524: negative regulation of neuron apoptotic process

ECO:0000314: direct assay evidence used in manual assertion

P

  • regulates_o_occurs_in:(CL:0000540)

Seeded From UniProt

complete

MOUSE:ANGP1

acts_upstream_of_or_within

GO:0043524: negative regulation of neuron apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:98664

P

  • regulates_o_occurs_in:(CL:0000540)

Seeded From UniProt

complete

MOUSE:NEST

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:TIE2

acts_upstream_of_or_within

GO:0043066: negative regulation of apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:108448

P

Seeded From UniProt

complete

MOUSE:TIE2

acts_upstream_of_or_within

GO:0033138: positive regulation of peptidyl-serine phosphorylation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:108448

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.