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PMID:19384922

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Citation

Yamamoto, A, Shofuda, T, Islam, MO, Nakamura, Y, Yamasaki, M, Okano, H and Kanemura, Y (2009) ABCB1 is predominantly expressed in human fetal neural stem/progenitor cells at an early development stage. J. Neurosci. Res. 87:2615-23

Abstract

ABCB1 is a human ABC transporter originally characterized by its ability to cause resistance to chemotherapy drugs in cancer cells, and later found to be functionally expressed in human neural stem/progenitor cells (NSPCs) in vitro. Here, we performed a detailed examination of ABCB1's expression on human NSPCs in vitro and in human fetal brain tissues, and analyzed the cellular properties of the human NSPCs expressing ABCB1. We confirmed that ABCB1 was expressed on the surface of human NSPCs, and its level correlated well with those of Nestin and CD133. The population of fluorescence-activated cell sorter-sorted human NSPCs expressing high levels of ABCB1 showed enrichment of proliferating cells, higher expression of 246 genes (e.g., RGS6, IGFBP7, GFAP, TNC, Hes1), and lower expression of 71 genes (e.g., STMN2, DLX5, BASP1, DCX, CD24) compared with human NSPCs expressing low or no ABCB1. In situ, ABCB1 was selectively expressed in cells in the ventricular or subventricular regions of lateral ventricles that expressed Nestin in human development. These findings suggest that ABCB1 is predominantly expressed in immature human fetal NSPCs in vitro and at early developmental stages in vivo, and that it may be a useful marker for human NSPCs.

Links

PubMed Online version:10.1002/jnr.22094

Keywords

Aged; Antigens, CD/metabolism; Biological Markers/analysis; Biological Markers/metabolism; Brain/cytology; Brain/embryology; Brain/metabolism; Cell Proliferation; Cells, Cultured; Fetus; Gene Expression Regulation, Developmental/genetics; Glycoproteins/metabolism; Humans; Intermediate Filament Proteins/metabolism; Lateral Ventricles/cytology; Lateral Ventricles/embryology; Lateral Ventricles/metabolism; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurogenesis/physiology; Neurons/cytology; Neurons/metabolism; P-Glycoprotein/metabolism; Peptides/metabolism; Spheroids, Cellular; Stem Cells/cytology; Stem Cells/metabolism; Telencephalon/cytology; Telencephalon/embryology; Telencephalon/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PROM1

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MDR1

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NEST

involved_in

GO:0000086: G2/M transition of mitotic cell cycle

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:NEST

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NEST

involved_in

GO:0072089: stem cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MDR1

involved_in

GO:0072089: stem cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MDR1

part_of

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MDR1

involved_in

GO:0000086: G2/M transition of mitotic cell cycle

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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