GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19376200

From GONUTS
Jump to: navigation, search
Citation

Jiang, Q, Li, MH, Papasian, CJ, Branigan, D, Xiong, ZG, Wang, JQ and Chu, XP (2009) Characterization of acid-sensing ion channels in medium spiny neurons of mouse striatum. Neuroscience 162:55-66

Abstract

Acid-sensing ion channels (ASICs) regulate synaptic activities and play important roles in neurodegenerative diseases. They are highly expressed in the striatum, where medium spiny neurons (MSNs) are a major population. Given that the properties of ASICs in MSNs are unknown, in this study, we characterized ASICs in MSNs of the mouse striatum. A rapid drop in extracellular pH induced transient inward currents in all MSNs. The pH value for half-maximal activation was 6.25, close to that obtained in homomeric ASIC1a channels. Based on psalmotoxin 1 and zinc sensitivity, ASIC1a (70.5% of neurons) and heteromeric ASIC1a-2 channels (29.5% of neurons) appeared responsible for the acid-induced currents in MSNs. ASIC currents were diminished in MSNs from ASIC1, but not ASIC2, null mice. Furthermore, a drop in pH induced calcium influx by activating homomeric ASIC1a channels. Activation of ASICs increased the membrane excitability of MSNs and lowering extracellular Ca2+ potentiated ASIC currents. Our data suggest that the homomeric ASIC1a channel represents a majority of the ASIC isoform in MSNs. The potential function of ASICs in the striatum requires further investigation.

Links

PubMed Online version:10.1016/j.neuroscience.2009.04.029

Keywords

Action Potentials/physiology; Amiloride/administration & dosage; Animals; Calcium/metabolism; Cells, Cultured; Chlorides/administration & dosage; Corpus Striatum/cytology; Corpus Striatum/drug effects; Corpus Striatum/physiology; Dose-Response Relationship, Drug; Extracellular Space/metabolism; Hydrogen-Ion Concentration; Intracellular Space/drug effects; Intracellular Space/metabolism; Membrane Potentials/physiology; Mice; Mice, Knockout; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurons/drug effects; Neurons/physiology; Sodium Channel Blockers/administration & dosage; Sodium Channels/genetics; Sodium Channels/metabolism; Spider Venoms/administration & dosage; Zinc Compounds/administration & dosage

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ASIC1

acts_upstream_of_or_within

GO:0034220: ion transmembrane transport

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2179834

P

Seeded From UniProt

complete

MOUSE:ASIC1

acts_upstream_of_or_within

GO:0042391: regulation of membrane potential

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2179834

P

Seeded From UniProt

complete

MOUSE:ASIC1

acts_upstream_of_or_within

GO:0070588: calcium ion transmembrane transport

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2179834

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.