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PMID:19343051

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Citation

Bies-Etheve, N, Pontier, D, Lahmy, S, Picart, C, Vega, D, Cooke, R and Lagrange, T (2009) RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family. EMBO Rep. 10:649-54

Abstract

Recent studies have identified a conserved WG/GW-containing motif, known as the Argonaute (AGO) hook, which is involved in the recruitment of AGOs to distinct components of the eukaryotic RNA silencing pathways. By using this motif as a model to detect new components in plant RNA silencing pathways, we identified SPT5-like, a plant-specific AGO4-interacting member of the nuclear SPT5 (Suppressor of Ty insertion 5) RNA polymerase (RNAP) elongation factor family that is characterized by the presence of a carboxy-terminal extension with more than 40 WG/GW motifs. Knockout SPT5-like mutants show a decrease in the accumulation of several 24-nt RNAs and hypomethylation at different loci revealing an implication in RNA-directed DNA methylation (RdDM). Here, we propose that SPT5-like emerged in plants as a facultative RNAP elongation factor. Its plant-specific origin and role in RdDM might reflect functional interactions with plant-specific RNA Pols required for RdDM.

Links

PubMed PMC2711833 Online version:10.1038/embor.2009.31

Keywords

Amino Acid Sequence; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/metabolism; Argonaute Proteins; Base Sequence; Chromosomal Proteins, Non-Histone/chemistry; Chromosomal Proteins, Non-Histone/metabolism; DNA Methylation; Models, Biological; Molecular Sequence Data; Mutation/genetics; Protein Binding; RNA, Plant/metabolism; RNA, Small Interfering/metabolism; Transcriptional Elongation Factors/chemistry; Transcriptional Elongation Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RDM3

involved_in

GO:0080188: gene silencing by RNA-directed DNA methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RDM3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9ZVD5

F

Seeded From UniProt

complete

ARATH:RDM3

acts_upstream_of_or_within

GO:0030422: siRNA processing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RDM3

acts_upstream_of_or_within

GO:0006306: DNA methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:F4JW79

F

Seeded From UniProt

complete


See also

References

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