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PMID:19309460

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Citation

Posé, D, Castanedo, I, Borsani, O, Nieto, B, Rosado, A, Taconnat, L, Ferrer, A, Dolan, L, Valpuesta, V and Botella, MA (2009) Identification of the Arabidopsis dry2/sqe1-5 mutant reveals a central role for sterols in drought tolerance and regulation of reactive oxygen species. Plant J. 59:63-76

Abstract

Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3-oxidosqualene, the precursor of cyclic triterpenoids. Here we report that the Arabidopsis drought hypersensitive/squalene epoxidase 1-5 (dry2/sqe1-5) mutant, identified by its extreme hypersensitivity to drought stress, has altered stomatal responses and root defects because of a point mutation in the SQUALENE EPOXIDASE 1 (SQE1) gene. GC-MS analysis indicated that the dry2/sqe1-5 mutant has altered sterol composition in roots but wild-type sterol composition in shoots, indicating an essential role for SQE1 in root sterol biosynthesis. Importantly, the stomatal and root defects of the dry2/sqe1-5 mutant are associated with altered production of reactive oxygen species. As RHD2 NADPH oxidase is de-localized in dry2/sqe1-5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, stomatal responses and drought tolerance.

Links

PubMed Online version:10.1111/j.1365-313X.2009.03849.x

Keywords

Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; DNA, Plant/genetics; Dehydration; Droughts; Gene Expression Regulation, Plant; Genetic Complementation Test; Mutation; NADPH Oxidase/metabolism; Oligonucleotide Array Sequence Analysis; Plant Roots/enzymology; Plant Roots/genetics; Plant Stomata/enzymology; Plant Stomata/genetics; Reactive Oxygen Species/metabolism; Squalene Monooxygenase/genetics; Squalene Monooxygenase/metabolism; Sterols/metabolism; Stress, Physiological

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ERG14

acts_upstream_of_or_within

GO:0016126: sterol biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ERG14

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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