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PMID:19309460
| Citation |
Posé, D, Castanedo, I, Borsani, O, Nieto, B, Rosado, A, Taconnat, L, Ferrer, A, Dolan, L, Valpuesta, V and Botella, MA (2009) Identification of the Arabidopsis dry2/sqe1-5 mutant reveals a central role for sterols in drought tolerance and regulation of reactive oxygen species. Plant J. 59:63-76 |
|---|---|
| Abstract |
Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3-oxidosqualene, the precursor of cyclic triterpenoids. Here we report that the Arabidopsis drought hypersensitive/squalene epoxidase 1-5 (dry2/sqe1-5) mutant, identified by its extreme hypersensitivity to drought stress, has altered stomatal responses and root defects because of a point mutation in the SQUALENE EPOXIDASE 1 (SQE1) gene. GC-MS analysis indicated that the dry2/sqe1-5 mutant has altered sterol composition in roots but wild-type sterol composition in shoots, indicating an essential role for SQE1 in root sterol biosynthesis. Importantly, the stomatal and root defects of the dry2/sqe1-5 mutant are associated with altered production of reactive oxygen species. As RHD2 NADPH oxidase is de-localized in dry2/sqe1-5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, stomatal responses and drought tolerance. |
| Links |
PubMed Online version:10.1111/j.1365-313X.2009.03849.x |
| Keywords |
Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; DNA, Plant/genetics; Dehydration; Droughts; Gene Expression Regulation, Plant; Genetic Complementation Test; Mutation; NADPH Oxidase/metabolism; Oligonucleotide Array Sequence Analysis; Plant Roots/enzymology; Plant Roots/genetics; Plant Stomata/enzymology; Plant Stomata/genetics; Reactive Oxygen Species/metabolism; Squalene Monooxygenase/genetics; Squalene Monooxygenase/metabolism; Sterols/metabolism; Stress, Physiological |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
ARATH:ERG14 |
acts_upstream_of_or_within |
GO:0016126: sterol biosynthetic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
ARATH:ERG14 |
acts_upstream_of_or_within |
GO:0009414: response to water deprivation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
See also
References
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