GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19304929

From GONUTS
Jump to: navigation, search
Citation

Klatte, M, Schuler, M, Wirtz, M, Fink-Straube, C, Hell, R and Bauer, P (2009) The analysis of Arabidopsis nicotianamine synthase mutants reveals functions for nicotianamine in seed iron loading and iron deficiency responses. Plant Physiol. 150:257-71

Abstract

Nicotianamine chelates and transports micronutrient metal ions in plants. It has been speculated that nicotianamine is involved in seed loading with micronutrients. A tomato (Solanum lycopersicum) mutant (chloronerva) and a tobacco (Nicotiana tabacum) transgenic line have been utilized to analyze the effects of nicotianamine loss. These mutants showed early leaf chlorosis and had sterile flowers. Arabidopsis (Arabidopsis thaliana) has four NICOTIANAMINE SYNTHASE (NAS) genes. We constructed two quadruple nas mutants: one had full loss of NAS function, was sterile, and showed a chloronerva-like phenotype (nas4x-2); another mutant, with intermediate phenotype (nas4x-1), developed chlorotic leaves, which became severe upon transition from the vegetative to the reproductive phase and upon iron (Fe) deficiency. Residual nicotianamine levels were sufficient to sustain the life cycle. Therefore, the nas4x-1 mutant enabled us to study late nicotianamine functions. This mutant had no detectable nicotianamine in rosette leaves of the reproductive stage but low nicotianamine levels in vegetative rosette leaves and seeds. Fe accumulated in the rosette leaves, while less Fe was present in flowers and seeds. Leaves, roots, and flowers showed symptoms of Fe deficiency, whereas leaves also showed signs of sufficient Fe supply, as revealed by molecular-physiological analysis. The mutant was not able to fully mobilize Fe to sustain Fe supply of flowers and seeds in the normal way. Thus, nicotianamine is needed for correct supply of seeds with Fe. These results are fundamental for plant manipulation approaches to modify Fe homeostasis regulation through alterations of NAS genes.

Links

PubMed PMC2675739 Online version:10.1104/pp.109.136374

Keywords

Alkyl and Aryl Transferases/genetics; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/metabolism; Azetidinecarboxylic Acid/analogs & derivatives; Azetidinecarboxylic Acid/metabolism; Basic Helix-Loop-Helix Transcription Factors/metabolism; Copper/metabolism; Copper/toxicity; Flowers/enzymology; Flowers/genetics; Flowers/metabolism; Homeostasis; Iron/metabolism; Iron/toxicity; Phenotype; Plant Leaves/enzymology; Plant Leaves/genetics; Plant Leaves/metabolism; Reproduction/genetics; Seeds/enzymology; Seeds/genetics; Seeds/metabolism; Stress, Physiological; Zinc/metabolism; Zinc/toxicity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:NAS4

enables

GO:0030410: nicotianamine synthase activity

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At1g09240
AGI_LocusCode:At5g04950
AGI_LocusCode:At5g56080

F

Seeded From UniProt

complete

ARATH:NAS4

acts_upstream_of_or_within

GO:0030418: nicotianamine biosynthetic process

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At1g09240
AGI_LocusCode:At5g04950
AGI_LocusCode:At5g56080

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.