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PMID:19303412

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Citation

Choi, YJ, Cho, SK, Hwang, KC, Park, C, Kim, JH, Park, SB, Hwang, S and Kim, JH (2009) Nm23-M5 mediates round and elongated spermatid survival by regulating GPX-5 levels. FEBS Lett. 583:1292-8

Abstract

Nucleoside diphosphate (NDP) kinases are involved in numerous regulatory processes associated with proliferation, development, and differentiation. Previously, we cloned a new member of the NDPK family from mouse, Nm23-M5, which encodes a 211-amino acid protein and has 86% identity to the human Nm23-H5 [Hwang, K.C., Ok, D.W., Hong, J.C., Kim, M.O. and Kim, J.H. (2003) Cloning, sequencing, and characterization of the murine Nm23-M5 gene during mouse spermatogenesis and spermiogenesis. Biochem. Biophys. Res. Commun. 306, 198-207]. To better understand Nm23-M5 function, we generated transgenic mice with reduced Nm23-M5 levels in vivo using a short hairpin RNA (shRNA) knock-down system. Nm23-M5 expression was markedly reduced, as indicated by Northern and Western blot analysis. Nm23-M5 shRNA transgenic mice exhibited reduced numbers of haploid cells. Furthermore, the antioxidant enzyme glutathione peroxidase 5 (GPX-5) is regulated by Nm23-M5 at the level of both expression and activity. These results reveal that expression of Nm23-M5 plays a critical role in spermiogenesis by increasing the cellular levels of GPX-5 to eliminate reactive oxygen species.

Links

PubMed Online version:10.1016/j.febslet.2009.03.023

Keywords

Animals; Base Sequence; Blotting, Northern; Blotting, Western; CHO Cells; Cell Survival/physiology; Cloning, Molecular; Cricetinae; Cricetulus; DNA Primers; Glutathione Peroxidase/metabolism; Male; Mice; Mice, Transgenic; NM23 Nucleoside Diphosphate Kinases/genetics; NM23 Nucleoside Diphosphate Kinases/physiology; Reactive Oxygen Species; Reverse Transcriptase Polymerase Chain Reaction; Spermatids/cytology; Spermatogenesis/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NDK5

located_in

GO:0036126: sperm flagellum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:NDK5

acts_upstream_of_or_within

GO:0007286: spermatid development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000018)
  • occurs_in:(EMAPA:18686)

Seeded From UniProt

complete


See also

References

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