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PMID:19298815

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Citation

Ruiz-Ederra, J and Verkman, AS (2009) Aquaporin-1-facilitated keratocyte migration in cell culture and in vivo corneal wound healing models. Exp. Eye Res. 89:159-65

Abstract

Aquaporin-1 (AQP1) water channels are expressed in corneal keratocytes, which become activated and migrate following corneal wounding. The purpose of this study was to investigate the role of AQP1 in keratocyte migration. Keratocyte primary cell cultures from wildtype and AQP1-null mice were compared, as well as keratocyte cultures from pig cornea in which AQP1 expression was modulated by RNAi knockdown and adenovirus-mediated overexpression. AQP1 expression was found in a plasma membrane pattern in corneal stromal and cultured keratocytes. Osmotic water permeability, as measured by calcein fluorescence quenching, was AQP1-dependent in cultured keratocytes, as was keratocyte migration following a scratch wound. Keratocyte migration in vivo was compared in wildtype and AQP1 knockout mice by histology and immunofluorescence of corneal sections at different times after partial-thickness corneal stromal debridement. AQP1 expression in keratocytes was increased by 24h after corneal debridement. Wound healing and keratocyte appearance near the wound margin were significantly reduced in AQP1 knockout mice, and the number of neutrophils was increased. These results implicate AQP1 water permeability as a new determinant of keratocyte migration in cornea.

Links

PubMed PMC3319399 Online version:10.1016/j.exer.2009.03.002

Keywords

Animals; Aquaporin 1/deficiency; Aquaporin 1/metabolism; Aquaporin 1/physiology; Cell Movement/physiology; Cells, Cultured; Cornea/injuries; Cornea/metabolism; Cornea/pathology; Corneal Stroma/metabolism; Endothelium, Corneal/metabolism; Eye Proteins/metabolism; Eye Proteins/physiology; Mice; Mice, Knockout; Osmolar Concentration; Sus scrofa; Wound Healing/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:AQP1

acts_upstream_of_or_within

GO:0042060: wound healing

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857807

P

  • occurs_in:(EMAPA:17602)

Seeded From UniProt

complete

MOUSE:AQP1

acts_upstream_of_or_within

GO:0042060: wound healing

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857807

P

Seeded From UniProt

complete

MOUSE:AQP1

acts_upstream_of_or_within

GO:0030335: positive regulation of cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857807

P

  • regulates_o_occurs_in:(EMAPA:17602)
  • regulates_o_results_in_movement_of:(CL:0000057)

Seeded From UniProt

complete


See also

References

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