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PMID:19286935

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Citation

Bu, Q, Li, H, Zhao, Q, Jiang, H, Zhai, Q, Zhang, J, Wu, X, Sun, J, Xie, Q, Wang, D and Li, C (2009) The Arabidopsis RING finger E3 ligase RHA2a is a novel positive regulator of abscisic acid signaling during seed germination and early seedling development. Plant Physiol. 150:463-81

Abstract

The phytohormone abscisic acid (ABA) is well known for its regulatory roles in integrating environmental constraints with the developmental programs of plants. Here, we characterize the biological function of the Arabidopsis (Arabidopsis thaliana) RING-H2 protein RHA2a in ABA signaling. The rha2a mutant is less sensitive to ABA than the wild type during seed germination and early seedling development, whereas transgenic plants overexpressing RHA2a are hypersensitive, indicating that RHA2a positively regulates ABA-mediated control of seed germination and early seedling development. Double mutant analyses of rha2a with several known ABA-insensitive mutants suggest that the action of RHA2a in ABA signaling is independent of that of the transcription factors ABI3, ABI4, and ABI5. We provide evidence showing that RHA2a also positively regulates plant responses to salt and osmotic stresses during seed germination and early seedling development. RHA2a is a functional E3 ubiquitin ligase, and its conserved RING domain is likely important for the biological function of RHA2a in ABA signaling. Together, these results suggest that the E3 ligase RHA2a is an important regulator of ABA signaling during seed germination and early seedling development.

Links

PubMed PMC2675735 Online version:10.1104/pp.109.135269

Keywords

Abscisic Acid/metabolism; Abscisic Acid/pharmacology; Arabidopsis/drug effects; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Basic-Leucine Zipper Transcription Factors/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Carrier Proteins/physiology; Germination/drug effects; Germination/genetics; Mutation; Osmotic Pressure; Seedling/growth & development; Seedling/metabolism; Seeds/drug effects; Seeds/growth & development; Seeds/metabolism; Signal Transduction; Sodium Chloride/pharmacology; Stress, Physiological; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:NAC19

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9ZT50

F

Seeded From UniProt

complete

ARATH:NAC55

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9ZT50

F

Seeded From UniProt

complete

ARATH:RHA2A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LDY8

F

Seeded From UniProt

complete

ARATH:RHA2A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9C932

F

Seeded From UniProt

complete

ARATH:RHA2A

acts_upstream_of_or_within

GO:0009789: positive regulation of abscisic acid-activated signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RHA2A

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RHA2A

enables

GO:0004842: ubiquitin-protein transferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:RHA2A

acts_upstream_of_or_within

GO:0047484: regulation of response to osmotic stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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