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PMID:19285469

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Citation

Jing, L, Lefebvre, JL, Gordon, LR and Granato, M (2009) Wnt signals organize synaptic prepattern and axon guidance through the zebrafish unplugged/MuSK receptor. Neuron 61:721-33

Abstract

Early during neuromuscular development, acetylcholine receptors (AChRs) accumulate at the center of muscle fibers, precisely where motor growth cones navigate and synapses eventually form. Here, we show that Wnt11r binds to the zebrafish unplugged/MuSK ectodomain to organize this central muscle zone. In the absence of such a zone, prepatterned AChRs fail to aggregate and, as visualized by live-cell imaging, growth cones stray from their central path. Using inducible unplugged/MuSK transgenes, we show that organization of the central muscle zone is dispensable for the formation of neural synapses, but essential for AChR prepattern and motor growth cone guidance. Finally, we show that blocking noncanonical dishevelled signaling in muscle fibers disrupts AChR prepatterning and growth cone guidance. We propose that Wnt ligands activate unplugged/MuSK signaling in muscle fibers to restrict growth cone guidance and AChR prepatterns to the muscle center through a mechanism reminiscent of the planar cell polarity pathway.

Links

PubMed PMC2671566 Online version:10.1016/j.neuron.2008.12.025

Keywords

Animals; Animals, Genetically Modified; Axons/physiology; Body Patterning/physiology; Embryo, Nonmammalian; Gene Expression Regulation, Developmental/genetics; Gene Expression Regulation, Developmental/physiology; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Hot Temperature; Immunoprecipitation/methods; RNA, Messenger/metabolism; Receptor Protein-Tyrosine Kinases/physiology; Signal Transduction/genetics; Synapses/physiology; Transfection/methods; Wnt Proteins/genetics; Wnt Proteins/metabolism; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism; Zebrafish Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A0R4IEH7

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:A0A0R4IJJ5

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:A0A0R4IZ74

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:A4JYH2

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:A4JYH3

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:Q5J1R8

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:Q5J1R9

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:Q7SY58

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:Q7ZZ92

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete

DANRE:Q8JFU3

involved_in

GO:0071340: skeletal muscle acetylcholine-gated channel clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070410-1

P

Seeded From UniProt

complete


See also

References

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