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PMID:19268593

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Citation

Krupnova, T, Sasabe, M, Ghebreghiorghis, L, Gruber, CW, Hamada, T, Dehmel, V, Strompen, G, Stierhof, YD, Lukowitz, W, Kemmerling, B, Machida, Y, Hashimoto, T, Mayer, U and Jürgens, G (2009) Microtubule-associated kinase-like protein RUNKEL needed [corrected] for cell plate expansion in Arabidopsis cytokinesis. Curr. Biol. 19:518-23

Abstract

Cytokinesis partitions the cytoplasm of dividing eukaryotic cells. In higher plants, a dynamic microtubule array--phragmoplast--mediates the formation of the partitioning membrane--cell plate--in a centrifugal fashion. This phragmoplast dynamic involves microtubule-associated proteins. Mutations in a novel Arabidopsis gene RUNKEL (RUK) result in cytokinesis defects caused by abnormal phragmoplast organization and arrested cell plate expansion. RUK encodes an essential cell-cycle-regulated 152 kDa protein with a putative serine/threonine kinase domain and a large microtubule-binding domain, both of which are largely conserved in uncharacterized proteins from protozoa, plants, and animals. RUK directly bound to microtubules in vitro and colocalized with mitotic preprophase band, spindle, and phragmoplast in vivo. An engineered RUK fusion protein that was degraded before telophase did not rescue the ruk mutant phenotype, demonstrating RUK action during cytokinesis. Both microtubule-binding domain and putative kinase domain were essential for RUK function. Surprisingly, RUK did not show kinase activity in vitro, and transgenically expressed "kinase-dead" RUK rescued the seedling lethality of ruk mutants. Our results suggest that RUK plays a regulatory, rather than catalytic, role in phragmoplast microtubule organization during cell plate expansion in cytokinesis.

Links

PubMed Online version:10.1016/j.cub.2009.02.021

Keywords

Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/metabolism; Cell Cycle/genetics; Cytokinesis/physiology; Gene Expression Regulation, Plant; Metaphase/physiology; Microtubule-Associated Proteins/metabolism; Microtubules/physiology; Mitotic Spindle Apparatus/genetics; Mitotic Spindle Apparatus/physiology; Mutation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RUK

NOT|enables

GO:0016301: kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:RUK

part_of

GO:0005819: spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RUK

part_of

GO:0009574: preprophase band

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RUK

part_of

GO:0009524: phragmoplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RUK

involved_in

GO:0000911: cytokinesis by cell plate formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RUK

involved_in

GO:0000914: phragmoplast assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RUK

enables

GO:0008017: microtubule binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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