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PMID:19263243

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Citation

Yu, CX, Jin, T, Chen, WW, Zhang, PJ, Liu, WW, Guan, HY, Zhang, J, Liu, QW and Jiang, AL (2009) Identification of Sp1-elements in the promoter region of human homeobox gene NKX3.1. Mol. Biol. Rep. 36:2353-60

Abstract

NKX3.1 is a prostate-specific homeobox gene related strongly to prostate development and prostate cancer. However, little is known about the mechanism for regulation of NKX3.1 in prostate cancer. With RT-PCR and western blot, we found that NKX3.1 expression was enhanced by over-expression of Sp1 at both the mRNA and protein levels in prostate cancer LNCaP cells. To identify the Sp1-elements in the promoter region of NKX3.1, a 521 bp-promoter of human NKX3.1 gene containing three possible Sp1-elements was cloned into the upstream of the luciferase reporter gene in pGL(3)-basic plasmid. With deletion mutation analysis, plasmid construction, EMSA and oligonucleotide decoy technique, two Sp1-elements which located between ?29 to ?43 and -60 to -46 of NKX3.1 gene were identified and proven to be functional elements. It will be important to further study on the functions and the regulatory mechanisms of Sp1 element in NKX3.1 gene expression.

Links

PubMed Online version:10.1007/s11033-009-9457-y

Keywords

Base Sequence; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Humans; Male; Molecular Sequence Data; Mutagenesis; Promoter Regions, Genetic; Prostatic Neoplasms/genetics; Prostatic Neoplasms/metabolism; Protein Binding; RNA, Messenger/genetics; RNA, Messenger/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sp1 Transcription Factor/genetics; Sp1 Transcription Factor/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SP1

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:Q99801)

Seeded From UniProt

complete

HUMAN:NKX31

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NKX31

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:NKX31

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:NKX31

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

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