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PMID:19251700

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Citation

Oshima, M, Mimura, J, Sekine, H, Okawa, H and Fujii-Kuriyama, Y (2009) SUMO modification regulates the transcriptional repressor function of aryl hydrocarbon receptor repressor. J. Biol. Chem. 284:11017-26

Abstract

The aryl hydrocarbon receptor (AhR) repressor (AhRR) inhibits the AhR activity. AhRR acts by competing with AhR for heterodimer formation with the AhR nuclear translocator (Arnt) and preventing the AhR.Arnt complex from binding the xenobiotic-responsive elements. Here, we report that AhRR has three evolutionarily conserved SUMOylation consensus sequences within its C-terminal repression domain and that Lys-542, Lys-583, and Lys-660 at the SUMOylation sites are modified by SUMO-1 in vivo. Arginine mutation of the three lysines results in a significant reduction of transcriptional repression activity. SUMOylation of the three lysine residues is important for the interaction between AhRR and ANKRA2, HDAC4, and HDAC5, which are important corepressors for AhRR. Arnt, a heterodimer partner for AhRR, markedly enhanced the SUMOylation of AhRR. AhRR, but not AhR, also significantly enhanced the SUMOylation of Arnt. The SUMOylation of both AhRR and Arnt is important for the efficient transcriptional repression activity of the AhRR/Arnt heterodimer.

Links

PubMed PMC2670107 Online version:10.1074/jbc.M808694200

Keywords

Amino Acid Sequence; Animals; Aryl Hydrocarbon Receptor Nuclear Translocator/metabolism; COS Cells; Cercopithecus aethiops; Gene Expression Regulation; HeLa Cells; Humans; Molecular Sequence Data; Protein Structure, Tertiary; Receptors, Aryl Hydrocarbon/metabolism; SUMO-1 Protein/metabolism; Sequence Homology, Amino Acid; Small Ubiquitin-Related Modifier Proteins/metabolism; Ubiquitins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ARNT

involved_in

GO:0033235: positive regulation of protein sumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AHRR

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AHRR

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:AHRR

involved_in

GO:0033235: positive regulation of protein sumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AHRR

involved_in

GO:0009410: response to xenobiotic stimulus

ECO:0000305: curator inference used in manual assertion

GO:0000122

P

Seeded From UniProt

complete

MOUSE:AHRR

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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