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PMID:19248824

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Citation

Yang, O, Popova, OV, Süthoff, U, Lüking, I, Dietz, KJ and Golldack, D (2009) The Arabidopsis basic leucine zipper transcription factor AtbZIP24 regulates complex transcriptional networks involved in abiotic stress resistance. Gene 436:45-55

Abstract

Soil salinity severely affects plant growth and agricultural productivity. AtbZIP24 encodes a bZIP transcription factor that is induced by salt stress in Arabidopsis thaliana but suppressed in the salt-tolerant relative Lobularia maritima. Transcriptional repression of AtbZIP24 using RNA interference improved salt tolerance in A. thaliana. Under non-stress growth conditions, transgenic A. thaliana lines with decreased AtbZIP24 expression activated the expression of stress-inducible genes involved in cytoplasmic ion homeostasis and osmotic adjustment: the Na(+) transporter AtHKT1, the Na(+)/H(+) antiporter AtSOS1, the aquaporin AtPIP2.1, and a glutamine synthetase. In addition, candidate target genes of AtbZIP24 with functions in plant growth and development were identified such as an argonaute (AGO1)-related protein and cyclophilin AtCYP19. The salt tolerance in transgenic plants correlated with reduced Na(+) accumulation in leaves. In vivo interaction of AtbZIP24 as a homodimer was shown using fluorescence energy transfer (FRET) with cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) as fused FRET pairs. Translational fusion of AtbZIP24 with GFP showed subcellular localization of the protein in nucleus and cytoplasm in plants grown under control conditions whereas in response to salt stress AtbZIP24 was preferentially targeted to the nucleus. It is concluded that AtbZIP24 is an important regulator of salt stress response in plants. The modification of transcriptional control by regulatory transcription factors provides a useful strategy for improving salt tolerance in plants.

Links

PubMed Online version:10.1016/j.gene.2009.02.010

Keywords

Adaptation, Physiological; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Basic-Leucine Zipper Transcription Factors/chemistry; Basic-Leucine Zipper Transcription Factors/genetics; Basic-Leucine Zipper Transcription Factors/metabolism; Basic-Leucine Zipper Transcription Factors/physiology; Cell Nucleus/metabolism; Cytoplasm/metabolism; Dimerization; Fluorescence Resonance Energy Transfer/methods; Gene Expression Regulation, Plant/drug effects; Gene Expression Regulation, Plant/genetics; Gene Regulatory Networks; Glucuronidase/genetics; Glucuronidase/metabolism; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Osmotic Pressure; Plants, Genetically Modified; Promoter Regions, Genetic/genetics; RNA Interference; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sodium Chloride/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4J5N9

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:F4J5N9

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:F4J5N9

acts_upstream_of_or_within

GO:0006970: response to osmotic stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4J5N9

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4J5N9

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4J5N9

NOT|involved_in

GO:0009409: response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4J5N9

acts_upstream_of_or_within

GO:0042538: hyperosmotic salinity response

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BZP24

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:BZP24

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:BZP24

enables

GO:0042803: protein homodimerization activity

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8GTS1

F

Seeded From UniProt

complete

ARATH:BZP24

involved_in

GO:1901001: negative regulation of response to salt stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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