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PMID:19247943

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Citation

Lorda-Diez, CI, Torre-Pérez, N, García-Porrero, JA, Hurle, JM and Montero, JA (2009) Expression of Id2 in the developing limb is associated with zones of active BMP signaling and marks the regions of growth and differentiation of the developing digits. Int. J. Dev. Biol. 53:1495-502

Abstract

Here we report the pattern of expression of inhibitor of DNA binding/differentiation factor 2 (Id2) in the developing chicken limb. We show that prior to stage 25, Id2 is expressed in the anterior and posterior mesoderm, the AER, and in the early skeletal chondrogenic aggregates. At more advanced stages of limb development Id2 is expressed in the undifferentiated subectodermal and interdigital mesenchyme and exhibits specific domains of expression in the growing digits. These expression domains were closely coincident with zones of activation of BMP-signaling as deduced from the distribution of phosphorylated SMADs 1/5/8. In micromass cultures transcripts of Id2 are associated with the nodules of chondrogenic differentiation. Expression of Id2 both in vivo and in vitro was up-regulated in experiments of BMP-gain-of-function and down-regulated after treatments with BMP-antagonists. Interestingly, interdigital application of TGFbeta2 transiently upregulates Id2 in coincidence with the inhibition of interdigital cell death and the commitment of the interdigital mesenchyme to form an ectopic digit. These data suggest that Id2 is a molecular mediator of BMP signaling acting in concert with the TGFbeta pathway during the formation of the digits.

Links

PubMed Online version:10.1387/ijdb.072415cl

Keywords

Animals; Body Patterning/genetics; Body Patterning/physiology; Bone Morphogenetic Protein 2/genetics; Bone Morphogenetic Protein 2/metabolism; Bone Morphogenetic Protein 7/genetics; Bone Morphogenetic Protein 7/metabolism; Bone Morphogenetic Proteins/genetics; Bone Morphogenetic Proteins/metabolism; Bone Morphogenetic Proteins/pharmacology; Cartilage/cytology; Cartilage/metabolism; Cell Differentiation/drug effects; Cell Differentiation/genetics; Cell Differentiation/physiology; Cell Proliferation/drug effects; Cells, Cultured; Chick Embryo; Chondrogenesis/drug effects; Extremities/embryology; Gene Expression Regulation, Developmental/drug effects; Immunohistochemistry; In Situ Hybridization; Inhibitor of Differentiation Protein 2/genetics; Inhibitor of Differentiation Protein 2/metabolism; Mesoderm/cytology; Mesoderm/metabolism; Microscopy, Confocal; Phosphorylation; Signal Transduction/genetics; Signal Transduction/physiology; Smad1 Protein/metabolism; Smad5 Protein/metabolism; Smad8 Protein/metabolism; Transforming Growth Factor beta/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

COTJA:Q3LSV1

involved_in

GO:0006915: apoptotic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

COTJA:Q3LSV1

involved_in

GO:0006915: apoptotic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:O73933

involved_in

GO:0003415: chondrocyte hypertrophy

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

CHICK:O73933

involved_in

GO:0035988: chondrocyte proliferation

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

CHICK:O73933

involved_in

GO:0043392: negative regulation of DNA binding

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

CHICK:O73933

enables

GO:0043425: bHLH transcription factor binding

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

CHICK:O73933

involved_in

GO:0051216: cartilage development

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

CHICK:O73933

involved_in

GO:0071773: cellular response to BMP stimulus

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q56I98

involved_in

GO:0030509: BMP signaling pathway

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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