GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19230664

From GONUTS
Jump to: navigation, search
Citation

Tanaka, H, Kitakura, S, De Rycke, R, De Groodt, R and Friml, J (2009) Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking. Curr. Biol. 19:391-7

Abstract

Endocytic vesicle trafficking is crucial for regulating activity and localization of plasma membrane components, but the process is still poorly genetically defined in plants. Membrane proteins of the PIN-FORMED (PIN) family exhibit polar localization in plant cells and facilitate cellular efflux of the plant hormone auxin, thereby regulating multiple developmental processes. PIN proteins undergo constitutive endocytosis and GNOM ARF GEF-dependent recycling, and their localization is under extensive regulation by developmental and environmental cues. We designed a fluorescence imaging-based screen to identify Arabidopsis thaliana mutants defective in internalization of proteins including PINs from the plasma membrane. We identified three mutant loci, BFA-visualized endocytic trafficking defective1 (ben1) through ben3 that do not efficiently accumulate PIN1-GFP in intracellular compartments after inhibition of recycling and secretion by fungal toxin brefeldin A (BFA). Fine mapping revealed that BEN1 encodes an ARF GEF vesicle trafficking regulator from the functionally uncharacterized BIG class. ben1 mutant has been previously implicated in pathogen response and shows cell polarity, BFA sensitivity, and growth defects. BEN1 is involved in endocytosis of plasma membrane proteins and localizes to early endocytic compartments distinct from GNOM-positive endosomes. Our results identify BEN1 as the ARF GEF mediating early endosomal traffic.

Links

PubMed Online version:10.1016/j.cub.2009.01.057

Keywords

ADP-Ribosylation Factors/genetics; ADP-Ribosylation Factors/metabolism; Alcohol Oxidoreductases/genetics; Alcohol Oxidoreductases/metabolism; Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Biological Transport/physiology; Cell Polarity; Endosomes/metabolism; Guanine Nucleotide Exchange Factors/genetics; Guanine Nucleotide Exchange Factors/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutation; Plant Roots/cytology; Plant Roots/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; trans-Golgi Network/metabolism; trans-Golgi Network/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:BIG5

located_in

GO:0005802: trans-Golgi network

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:BIG5

acts_upstream_of_or_within

GO:0016192: vesicle-mediated transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BIG5

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.