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PMID:19223514

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Citation

Gallois, JL, Guyon-Debast, A, Lécureuil, A, Vezon, D, Carpentier, V, Bonhomme, S and Guerche, P (2009) The Arabidopsis proteasome RPT5 subunits are essential for gametophyte development and show accession-dependent redundancy. Plant Cell 21:442-59

Abstract

We investigated the role of the ubiquitin proteasome system (UPS), which allows proteins to be selectively degraded, during gametophyte development in Arabidopsis thaliana. Three mutant alleles altering the UPS were isolated in the Wassilewskija (Ws) accession: they affect the Regulatory Particle 5a (RPT5a) gene, which (along with RPT5b) encodes one of the six AAA-ATPases of the proteasome regulatory particle. In the heterozygous state, all three mutant alleles displayed 50% pollen lethality, suggesting that RPT5a is essential for male gametophyte development. However, a fourth mutant in the Columbia (Col) accession did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants showed a complete male and female gametophyte lethal phenotype in a Col background, indicating that RPT5 subunits are essential for both gametophytic phases. Mitotic divisions were affected in double mutant gametophytes correlating with an absence of the proteasome-dependent cyclinA3 degradation. Finally, we show that RPT5b expression is highly increased when proteasome functioning is defective, allowing complementation of the rpt5a mutation. In conclusion, RPT5 subunits are not only essential for both male and female gametophyte development but also display accession-dependent redundancy and are crucial in cell cycle progression.

Links

PubMed PMC2660631 Online version:10.1105/tpc.108.062372

Keywords

Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/physiology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Feedback, Physiological; Mitosis/genetics; Mitosis/physiology; Molecular Sequence Data; Pollen/genetics; Pollen/growth & development; Proteasome Endopeptidase Complex/physiology; Protein Subunits/genetics; Protein Subunits/metabolism; Protein Subunits/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PS6AB

acts_upstream_of_or_within

GO:0010498: proteasomal protein catabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At3g05530

P

Seeded From UniProt

complete

ARATH:PS6AB

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At3g05530

P

Seeded From UniProt

complete

ARATH:PS6AB

acts_upstream_of_or_within

GO:0009553: embryo sac development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At3g05530

P

Seeded From UniProt

complete

ARATH:PS6AA

acts_upstream_of_or_within

GO:0010498: proteasomal protein catabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At1g09100

P

Seeded From UniProt

complete

ARATH:PS6AA

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At1g09100

P

Seeded From UniProt

complete

ARATH:PS6AA

acts_upstream_of_or_within

GO:0009553: embryo sac development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1g09100

P

Seeded From UniProt

complete


See also

References

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