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PMID:19223001

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Citation

Prabhakar, V, Löttgert, T, Gigolashvili, T, Bell, K, Flügge, UI and Häusler, RE (2009) Molecular and functional characterization of the plastid-localized Phosphoenolpyruvate enolase (ENO1) from Arabidopsis thaliana. FEBS Lett. 583:983-91

Abstract

The Arabidopsis thaliana gene At1g74030 codes for a putative plastid phosphoenolpyruvate (PEP) enolase (ENO1). The recombinant ENO1 protein exhibited enolase activity and its kinetic properties were determined. ENO1 is localized to plastids and expressed in most heterotrophic tissues including trichomes and non-root-hair cells, but not in the mesophyll of leaves. Two T-DNA insertion eno1 mutants exhibited distorted trichomes and reduced numbers of root hairs as the only visible phenotype. The essential role of ENO1 in PEP provision for anabolic processes within plastids, such as the shikimate pathway, is discussed with respect to plastid transporters, such as the PEP/phosphate translocator.

Links

PubMed Online version:10.1016/j.febslet.2009.02.017

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Cloning, Molecular; Enzyme Activation; Kinetics; Organ Specificity/genetics; Phosphopyruvate Hydratase/genetics; Phosphopyruvate Hydratase/isolation & purification; Phosphopyruvate Hydratase/metabolism; Plant Roots/genetics; Plant Roots/metabolism; Plants, Genetically Modified; Plastids/metabolism; Tissue Distribution

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ENO1

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ENO1

acts_upstream_of_or_within

GO:0010090: trichome morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ENO1

enables

GO:0004634: phosphopyruvate hydratase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ENO3

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ENO3

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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