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PMID:19218514

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Citation

Cruz, S, Shiao, JC, Liao, BK, Huang, CJ and Hwang, PP (2009) Plasma membrane calcium ATPase required for semicircular canal formation and otolith growth in the zebrafish inner ear. J. Exp. Biol. 212:639-47

Abstract

Fish otoliths consist of >90% calcium carbonate, the accretion of which depends on acellular endolymph. This study confirms the presence of plasma membrane calcium ATPase 1a isoform (Atp2b1a) in the auditory and vestibular system of a teleost fish. As shown by in situ hybridization, zebrafish atp2b1a is expressed mainly in larval otic placode and lateral-line neuromast as well as in the hair cells within the adult zebrafish inner ear chamber. Zebrafish atp2b1a knockdown by antisense morpholinos reduced the number of hair cells and produced malformation of semicircular canals and smaller otoliths. These defects coincide with unbalanced body orientation. The formation of smaller otoliths in atp2b1a morphants may stem from an impairment of calcium supply in the endolymph. However, otolith formation persists in most morphants, suggesting that other zebrafish Atp2b isoforms or paracellular pathways may also transport calcium into the endolymph. These results suggest that Atp2b1a plays an important role for normal development of the auditory and vestibular system as well as calcium transport in the inner ear of zebrafish.

Links

PubMed Online version:10.1242/jeb.022798

Keywords

Animals; Calcium Signaling/genetics; Calcium-Transporting ATPases/genetics; Calcium-Transporting ATPases/metabolism; Calcium-Transporting ATPases/physiology; Cell Membrane/enzymology; Cloning, Molecular; Otolithic Membrane/cytology; Otolithic Membrane/embryology; Phenotype; Phylogeny; Protein Isoforms/genetics; Protein Isoforms/metabolism; Protein Isoforms/physiology; RNA, Messenger/metabolism; Semicircular Canals/cytology; Semicircular Canals/embryology; Sequence Analysis, Protein; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism; Zebrafish Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A1L214

involved_in

GO:0048919: posterior lateral line neuromast development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:A1L214

involved_in

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:A1L214

involved_in

GO:0060119: inner ear receptor cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:F6PDY7

involved_in

GO:0048919: posterior lateral line neuromast development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:F6PDY7

involved_in

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:F6PDY7

involved_in

GO:0060119: inner ear receptor cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q0V934

involved_in

GO:0048919: posterior lateral line neuromast development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q0V934

involved_in

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q0V934

involved_in

GO:0060119: inner ear receptor cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q1RM46

involved_in

GO:0048919: posterior lateral line neuromast development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q1RM46

involved_in

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q1RM46

involved_in

GO:0060119: inner ear receptor cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q569P8

involved_in

GO:0048919: posterior lateral line neuromast development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q569P8

involved_in

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q569P8

involved_in

GO:0060119: inner ear receptor cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q5RGZ4

involved_in

GO:0048919: posterior lateral line neuromast development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q5RGZ4

involved_in

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q5RGZ4

involved_in

GO:0060119: inner ear receptor cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q6DGN2

involved_in

GO:0048919: posterior lateral line neuromast development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q6DGN2

involved_in

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete

DANRE:Q6DGN2

involved_in

GO:0060119: inner ear receptor cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100414-3

P

Seeded From UniProt

complete


See also

References

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