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PMID:19218459

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Citation

Benitez-Alfonso, Y, Cilia, M, San Roman, A, Thomas, C, Maule, A, Hearn, S and Jackson, D (2009) Control of Arabidopsis meristem development by thioredoxin-dependent regulation of intercellular transport. Proc. Natl. Acad. Sci. U.S.A. 106:3615-20

Abstract

Cell-to-cell transport in plants occurs through cytoplasmic channels called "plasmodesmata" and is regulated by developmental and environmental factors. Callose deposition modulates plasmodesmal transport in vivo, but little is known about the mechanisms that regulate this process. Here we report a genetic approach to identify mutants affecting plasmodesmal transport. We isolated 5 mutants, named gfp arrested trafficking (gat), affected in GFP unloading from the phloem into the meristem. gat1 mutants were seedling lethal and carried lesions in an m-type thioredoxin that is expressed in non-green plastids of meristems and organ primordia. Callose and hydrogen peroxide accumulated in gat1 mutants, and WT plants subjected to oxidative conditions phenocopied the gat1 trafficking defects. Ectopic expression of GAT1 in mature leaves increased plasmodesmal permeability and led to a delay in senescence and flowering time. We propose a role for the GAT1 thioredoxin in the redox regulation of callose deposition and symplastic permeability that is essential for meristem maintenance in Arabidopsis.

Links

PubMed PMC2651306 Online version:10.1073/pnas.0808717106

Keywords

Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Biological Transport; Gene Expression Regulation, Plant; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Meristem/genetics; Meristem/growth & development; Meristem/metabolism; Phenotype; Plants, Genetically Modified; Seedling/genetics; Seedling/growth & development; Seedling/metabolism; Thioredoxins/genetics; Thioredoxins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TRXM3

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TRXM3

involved_in

GO:0048509: regulation of meristem development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TRXM3

involved_in

GO:0045454: cell redox homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TRXM3

involved_in

GO:0010647: positive regulation of cell communication

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TRXM3

involved_in

GO:0010497: plasmodesmata-mediated intercellular transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TRXM3

part_of

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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