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PMID:19214217

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Citation

Wang, L, Tsuda, K, Sato, M, Cohen, JD, Katagiri, F and Glazebrook, J (2009) Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae. PLoS Pathog. 5:e1000301

Abstract

Salicylic acid (SA)-induced defense responses are important factors during effector triggered immunity and microbe-associated molecular pattern (MAMP)-induced immunity in plants. This article presents evidence that a member of the Arabidopsis CBP60 gene family, CBP60g, contributes to MAMP-triggered SA accumulation. CBP60g is inducible by both pathogen and MAMP treatments. Pseudomonas syringae growth is enhanced in cbp60g mutants. Expression profiles of a cbp60g mutant after MAMP treatment are similar to those of sid2 and pad4, suggesting a defect in SA signaling. Accordingly, cbp60g mutants accumulate less SA when treated with the MAMP flg22 or a P. syringae hrcC strain that activates MAMP signaling. MAMP-induced production of reactive oxygen species and callose deposition are unaffected in cbp60g mutants. CBP60g is a calmodulin-binding protein with a calmodulin-binding domain located near the N-terminus. Calmodulin binding is dependent on Ca(2+). Mutations in CBP60g that abolish calmodulin binding prevent complementation of the SA production and bacterial growth defects of cbp60g mutants, indicating that calmodulin binding is essential for the function of CBP60g in defense signaling. These studies show that CBP60g constitutes a Ca(2+) link between MAMP recognition and SA accumulation that is important for resistance to P. syringae.

Links

PubMed PMC2633612 Online version:10.1371/journal.ppat.1000301

Keywords

Analysis of Variance; Arabidopsis/genetics; Arabidopsis/immunology; Arabidopsis/metabolism; Arabidopsis/microbiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/physiology; Calmodulin/metabolism; Calmodulin-Binding Proteins/genetics; Calmodulin-Binding Proteins/physiology; Cluster Analysis; Gene Expression Profiling; Glucans/metabolism; Immunity, Innate; Intramolecular Transferases/metabolism; Mutagenesis, Insertional; Mutagenesis, Site-Directed; Oligonucleotide Array Sequence Analysis; Plant Diseases/genetics; Plant Diseases/immunology; Plant Diseases/microbiology; Pseudomonas syringae/physiology; Reactive Oxygen Species/metabolism; Salicylic Acid/metabolism; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:CB60G

enables

GO:0005516: calmodulin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CB60G

acts_upstream_of_or_within

GO:0009617: response to bacterium

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CB60G

acts_upstream_of_or_within

GO:0002237: response to molecule of bacterial origin

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CB60G

acts_upstream_of_or_within

GO:0042742: defense response to bacterium

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CB60G

acts_upstream_of_or_within

GO:0009697: salicylic acid biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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