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PMID:19213599

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Citation

Duran, MB, Rahman, A, Colten, M and Brazill, D (2009) Dictyostelium discoideum paxillin regulates actin-based processes. Protist 160:221-32

Abstract

Paxillin is a key player in integrating the actin cytoskeleton with adhesion, and thus is essential to numerous cellular processes, including proliferation, differentiation, and migration in animal cells. PaxB, the Dictyostelium discoideum orthologue of paxillin, has been shown to be important for adhesion and development, much like its mammalian counterpart. Here, we use the overproduction of PaxB to gain better insight into its role in regulating the actin cytoskeleton and adhesion. We find that PaxB-overexpressing (PaxBOE) cells can aggregate and form mounds normally, but are blocked in subsequent development. This arrest can be rescued by addition of wild-type cells, indicating a non-cell autonomous role for PaxB. PaxBOE cells also have alterations in several actin-based processes, including adhesion, endocytosis, motility, and chemotaxis. PaxBOE cells exhibit an EDTA-sensitive increase in cell-cell cohesion, suggesting that PaxB-mediated adhesion is Ca(2+) or Mg(2+) dependent. Interestingly, cells overexpressing paxB are less adhesive to the substratum. In addition, PaxBOE cells display decreased motility under starved conditions, decreased endocytosis, and are unable to efficiently chemotax up a folate gradient. Taken together, the data suggest that proper expression of PaxB is vital for the regulation of development and actin-dependent processes.

Links

PubMed PMC2743336 Online version:10.1016/j.protis.2008.09.005

Keywords

Actins/genetics; Actins/metabolism; Animals; Cell Adhesion; Chemotaxis; Cytoskeleton/genetics; Cytoskeleton/metabolism; Dictyostelium/genetics; Dictyostelium/growth & development; Dictyostelium/physiology; Endocytosis; Gene Expression; Paxillin/genetics; Paxillin/metabolism; Protozoan Proteins/genetics; Protozoan Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:PAXB

acts_upstream_of_or_within

GO:0048870: cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0040011: locomotion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0031589: cell-substrate adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0031154: culmination involved in sorocarp development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0016477: cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0016339: calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0006897: endocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0043326: chemotaxis to folate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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