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PMID:19211705

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Citation

Adamczyk, BJ and Fernandez, DE (2009) MIKC* MADS domain heterodimers are required for pollen maturation and tube growth in Arabidopsis. Plant Physiol. 149:1713-23

Abstract

MADS box genes encode transcription factors that play important regulatory roles at various stages in plant development. Transcripts encoding the MIKC*-type (for MADS DNA-binding domain, Intervening domain, Keratin-like domain, and C-terminal domain) factors, a divergent clade, are enriched in mature pollen. Previous studies have shown that these proteins bind DNA as heterodimers, which form between S- and P-class MIKC* proteins. In this study, Arabidopsis (Arabidopsis thaliana) pollen with little or no MIKC* activity was produced by combining strong loss-of-function alleles of the S-class proteins AGAMOUS-LIKE66 (AGL66) and AGL104. Double mutant plants produce pollen but have severely reduced fertility due to reduced pollen viability, delayed germination, and aberrant pollen tube growth. Microarray analysis of the mutant pollen revealed that the loss of MIKC* regulation has a major impact on pollen gene expression. Pollen competition assays involving various combinations of AGL65, AGL66, AGL104, and AGL94 mutant alleles provided genetic evidence that at least three heterodimers (AGL30-AGL104, AGL65-AGL104, and AGL30-AGL66) form and function in at least a partially redundant fashion in pollen. Analyses of transcript abundance in wild-type and mutant pollen indicated that AGL65-containing complexes are likely to be more abundant than the others and that accumulation of AGL30 and AGL94 transcripts increases in response to reductions in MIKC* activity. These results were combined to create a model to describe MIKC* heterodimer contributions in pollen.

Links

PubMed PMC2663741 Online version:10.1104/pp.109.135806

Keywords

Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/ultrastructure; Arabidopsis Proteins/metabolism; Gene Expression Regulation, Plant; Genes, Plant; Germ Cells/metabolism; MADS Domain Proteins/metabolism; Models, Biological; Pollen/cytology; Pollen/genetics; Pollen/growth & development; Pollen/ultrastructure; Protein Multimerization; RNA, Messenger/genetics; RNA, Messenger/metabolism; Reverse Transcriptase Polymerase Chain Reaction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:AGL30

involved_in

GO:0080092: regulation of pollen tube growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGL30

involved_in

GO:0010152: pollen maturation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGL30

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1PFC2

F

Seeded From UniProt

complete

ARATH:AGL30

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LM46

F

Seeded From UniProt

complete

ARATH:AGL30

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G18750
AGI_LocusCode:AT1G22130

P

Seeded From UniProt

complete

ARATH:AGL66

involved_in

GO:0080092: regulation of pollen tube growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGL66

involved_in

GO:0010152: pollen maturation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGL66

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1PFA4

F

Seeded From UniProt

complete

ARATH:AGL66

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G18750
AGI_LocusCode:AT1G22130

P

Seeded From UniProt

complete

ARATH:AGL65

involved_in

GO:0080092: regulation of pollen tube growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGL65

involved_in

GO:0010152: pollen maturation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGL65

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LM46

F

Seeded From UniProt

complete

ARATH:AGL65

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G18750
AGI_LocusCode:AT1G22130

P

Seeded From UniProt

complete

ARATH:AG104

involved_in

GO:0080092: regulation of pollen tube growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AG104

involved_in

GO:0010152: pollen maturation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AG104

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1PFA4

F

Seeded From UniProt

complete

ARATH:AG104

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q7X9I0

F

Seeded From UniProt

complete

ARATH:AG104

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G18750
AGI_LocusCode:At1G77980

P

Seeded From UniProt

complete


See also

References

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