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PMID:19211552

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Citation

Zoeller, JJ, Pimtong, W, Corby, H, Goldoni, S, Iozzo, AE, Owens, RT, Ho, SY and Iozzo, RV (2009) A central role for decorin during vertebrate convergent extension. J. Biol. Chem. 284:11728-37

Abstract

Decorin, an archetypal member of the small leucine-rich proteoglycan gene family, regulates collagen fibrillogenesis and cell growth. To further explore its biological function, we examined the role of Decorin during zebrafish development. Zebrafish Decorin is a chondroitin sulfate proteoglycan that exhibits a high degree of conservation with its mammalian counterpart and displays a unique spatiotemporal expression pattern. Morpholino-mediated knockdown of zebrafish decorin identified a developmental role during medial-lateral convergence and anterior-posterior extension of the body plan, as well as in craniofacial cartilage formation. decorin morphants displayed a pronounced shortening of the head-to-tail axis as well as compression, flattening, and extension of the jaw cartilages. The morphant phenotype was efficiently rescued by zebrafish decorin mRNA. Unexpectedly, microinjection of excess zebrafish decorin mRNA or proteoglycan/protein core into one-cell stage embryos caused cyclopia. The morphant and overexpression phenotype represent a convergent extension defect. Our results indicate a central function for Decorin during early embryogenesis.

Links

PubMed PMC2670176 Online version:10.1074/jbc.M808991200

Keywords

Animals; Cartilage/metabolism; Decorin; Developmental Biology/methods; Extracellular Matrix Proteins/metabolism; Extracellular Matrix Proteins/physiology; Gene Expression Regulation; Humans; Immunohistochemistry/methods; Models, Biological; Phenotype; Phylogeny; Proteoglycans/metabolism; Proteoglycans/physiology; RNA/metabolism; RNA, Messenger/metabolism; Time Factors; Vertebrates; Zebrafish

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:Q5RI45

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-6

P

Seeded From UniProt

complete

DANRE:Q5RI45

involved_in

GO:0050654: chondroitin sulfate proteoglycan metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:Q5RI45

involved_in

GO:0060028: convergent extension involved in axis elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-6

P

Seeded From UniProt

complete

DANRE:Q5RI45

involved_in

GO:0060028: convergent extension involved in axis elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-5

P

Seeded From UniProt

complete

DANRE:Q803T1

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-6

P

Seeded From UniProt

complete

DANRE:Q803T1

involved_in

GO:0050654: chondroitin sulfate proteoglycan metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:Q803T1

involved_in

GO:0060028: convergent extension involved in axis elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-6

P

Seeded From UniProt

complete

DANRE:Q803T1

involved_in

GO:0060028: convergent extension involved in axis elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-5

P

Seeded From UniProt

complete

DANRE:Q9DE01

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-6

P

Seeded From UniProt

complete

DANRE:Q9DE01

involved_in

GO:0050654: chondroitin sulfate proteoglycan metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:Q9DE01

involved_in

GO:0060028: convergent extension involved in axis elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-6

P

Seeded From UniProt

complete

DANRE:Q9DE01

involved_in

GO:0060028: convergent extension involved in axis elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-090513-5

P

Seeded From UniProt

complete


See also

References

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