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PMID:19210716

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Citation

Lin, YF, Liang, HM, Yang, SY, Boch, A, Clemens, S, Chen, CC, Wu, JF, Huang, JL and Yeh, KC (2009) Arabidopsis IRT3 is a zinc-regulated and plasma membrane localized zinc/iron transporter. New Phytol. 182:392-404

Abstract

ZIP transporters (ZRT, IRT-like proteins) are involved in the transport of iron (Fe), zinc (Zn) and other divalent metal cations. The expression of IRT3, a ZIP transporter, is higher in the Zn/cadmium (Cd) hyperaccumulator Arabidopsis halleri than is that of its ortholog in Arabidopsis thaliana, which implies a positive association of its expression with Zn accumulation in A. halleri. IRT3 genes from both A. halleri and A. thaliana functionally complemented the Zn uptake mutant Spzrt1 in Schizosaccharomyces pombe; and Zn uptake double mutant zrt1zrt2, Fe-uptake mutant fet3fet4 and conferred Zn and Fe uptake activity in Saccharomyces cerevisiae. By contrast, the manganese (Mn) uptake mutant smf1 phenotypes were not rescued. Insufficient Cd uptake for toxicity was found. Expression of IRT3-green fluorescent protein (GFP) fusion proteins in Arabidopsis root protoplasts indicated localization of both IRT3 proteins in the plasma membrane. Overexpressing AtIRT3 in A. thaliana led to increased accumulation of Zn in the shoot and Fe in the root of transgenic lines. Therefore, IRT3 functions as a Zn and Fe-uptake transporter in Arabidopsis.

Links

PubMed Online version:10.1111/j.1469-8137.2009.02766.x

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Biological Transport/genetics; Cadmium/metabolism; Cation Transport Proteins/genetics; Cation Transport Proteins/metabolism; Cell Membrane/genetics; Gene Expression; Genes, Plant; Iron/metabolism; Manganese/metabolism; Membrane Transport Proteins/genetics; Zinc/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:IRT3

enables

GO:0005381: iron ion transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:IRT3

enables

GO:0005385: zinc ion transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:IRT3

acts_upstream_of_or_within

GO:0006829: zinc ion transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:IRT3

acts_upstream_of_or_within

GO:0006826: iron ion transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:IRT3

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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