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PMID:19192391

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Citation

Li, Q, Sun, X, Wu, J, Lin, Z and Luo, Y (2009) PKD2 interacts with Lck and regulates NFAT activity in T cells. BMB Rep 42:35-40

Abstract

Protein kinase D2 (PKD2) is a member of the PKD serine/threonine protein kinase family that has been implicated in the regulation of a variety of cellular processes including proliferation, survival, protein trafficking and immune response. In the present study, we report a novel interaction between PKD2 and Lck, a member of the Src tyrosine protein kinase family that is predominantly expressed in T cells. This interaction involved the C-terminal kinase domains of both PKD2 and Lck. Moreover, co-expression of Lck enhanced the tyrosine phosphorylation of PKD2 and increased its kinase activity. Finally, we report that PKD2 enhanced T cell receptor (TCR)-induced nuclear factor of T cell (NFAT) activity in Jurkat T cells. These results suggested that Lck regulated the activity of PKD2 by tyrosine phosphorylation, which in turn may have modulated the physiological functions of PKD2 during TCR-induced T cell activation. [BMB reports 2009; 42(1): 35-40].

Links

PubMed

Keywords

Genes, Reporter; Humans; Jurkat Cells; Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/chemistry; Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/metabolism; NFATC Transcription Factors/metabolism; Phosphotyrosine/metabolism; Protein Binding; Protein Interaction Mapping; Protein Kinases/chemistry; Protein Kinases/metabolism; Receptors, Antigen, T-Cell/metabolism; T-Lymphocytes/enzymology; T-Lymphocytes/metabolism; Transcription Factor AP-1/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:LCK

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9BZL6

F

Seeded From UniProt

complete

HUMAN:KPCD2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P06239

F

Seeded From UniProt

complete

HUMAN:KPCD2

involved_in

GO:0051091: positive regulation of DNA-binding transcription factor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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