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PMID:19174463

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Citation

Grob, A, Roussel, P, Wright, JE, McStay, B, Hernandez-Verdun, D and Sirri, V (2009) Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis. J. Cell. Sci. 122:489-98

Abstract

Sirtuins, also designated class III histone deacetylases, are implicated in the regulation of cell division, apoptosis, DNA damage repair, genomic silencing and longevity. The nucleolar Sirtuin7 (SIRT7) was reported to be involved in the regulation of ribosomal gene (rDNA) transcription, but there are no data concerning the regulation of SIRT7 during the cell cycle. Here we have analyzed the behavior of endogenous SIRT7 during mitosis, while rDNA transcription is repressed. SIRT7 remains associated with nucleolar organizer regions, as does the RNA polymerase I machinery. SIRT7 directly interacts with the rDNA transcription factor UBF. Moreover, SIRT7 is phosphorylated via the CDK1-cyclin B pathway during mitosis and dephosphorylated by a phosphatase sensitive to okadaic acid at the exit from mitosis before onset of rDNA transcription. Interestingly, dephosphorylation events induce a conformational modification of the carboxy-terminal region of SIRT7 before the release of mitotic repression of rDNA transcription. As SIRT7 activity is required to resume rDNA transcription in telophase, we propose that this conformational modification regulates onset of rDNA transcription.

Links

PubMed PMC2714433 Online version:10.1242/jcs.042382

Keywords

CDC2 Protein Kinase/metabolism; Cell Cycle; Cyclin B/metabolism; DNA, Ribosomal/biosynthesis; DNA, Ribosomal/genetics; HeLa Cells; Humans; Metabolic Networks and Pathways/physiology; Mitosis; Nucleolus Organizer Region/enzymology; Phosphoric Monoester Hydrolases/metabolism; Phosphorylation/physiology; Pol1 Transcription Initiation Complex Proteins/metabolism; Protein Processing, Post-Translational; Sirtuins/metabolism; Transcriptional Activation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:UBF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9NRC8

F

Seeded From UniProt

complete

HUMAN:SIR7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17480

F

Seeded From UniProt

complete

HUMAN:SIR7

located_in

GO:0005731: nucleolus organizer region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SIR7

part_of

GO:0005731: nucleolus organizer region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SIR7

involved_in

GO:0007072: positive regulation of transcription involved in exit from mitosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SIR7

involved_in

GO:0009303: rRNA transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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