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PMID:19170886

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Citation

Muller, C, Bang, IS, Velayudhan, J, Karlinsey, J, Papenfort, K, Vogel, J and Fang, FC (2009) Acid stress activation of the sigma(E) stress response in Salmonella enterica serovar Typhimurium. Mol. Microbiol. 71:1228-38

Abstract

The alternative sigma factor sigma(E) is activated by unfolded outer membrane proteins (OMPs) and plays an essential role in Salmonella pathogenesis. The canonical pathway of sigma(E) activation in response to envelope stress involves sequential proteolysis of the anti-sigma factor RseA by the PDZ proteases DegS and RseP. Here we show that sigma(E) in Salmonella enterica sv. Typhimurium can also be activated by acid stress. A sigma(E)-deficient mutant exhibits increased susceptibility to acid pH and reduced survival in an acidified phagosomal vacuole. Acid activation of sigma(E)-dependent gene expression is independent of the unfolded OMP signal or the DegS protease but requires processing of RseA by RseP. The RseP PDZ domain is indispensable for acid induction, suggesting that acid stress may disrupt an inhibitory interaction between RseA and the RseP PDZ domain to allow RseA proteolysis in the absence of antecedent action of DegS. These observations demonstrate a novel environmental stimulus and activation pathway for the sigma(E) regulon that appear to be critically important during Salmonella-host cell interactions.

Links

PubMed Online version:10.1111/j.1365-2958.2009.06597.x

Keywords

Acids/metabolism; Animals; Bacterial Proteins/metabolism; Cell Line; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Mice; Mutation; RNA, Bacterial/metabolism; Regulon; Salmonella typhimurium/genetics; Salmonella typhimurium/metabolism; Sigma Factor/metabolism; Stress, Physiological; Vacuoles/microbiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SALT1:RSEP

involved_in

GO:0006508: proteolysis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:RSEP

involved_in

GO:0009266: response to temperature stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:RSEP

involved_in

GO:0097533: cellular stress response to acid chemical

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:RSEA

involved_in

GO:0097533: cellular stress response to acid chemical

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:RPOE

involved_in

GO:0009266: response to temperature stimulus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:RPOE

involved_in

GO:0097533: cellular stress response to acid chemical

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:DEGS

involved_in

GO:0006508: proteolysis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:DEGS

involved_in

GO:0009266: response to temperature stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALT1:DEGS

NOT|involved_in

GO:0097533: cellular stress response to acid chemical

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SALTY:RSEP

Contributes to

GO:0016020: membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Figure 5: Show the rseP mutation changing the function of acid induction of sigma factor e

complete


See also

References

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