PMID:19168682

Citation	Patalano, S, Mihailovich, M, Belacortu, Y, Paricio, N and Gebauer, F (2009) Dual sex-specific functions of Drosophila Upstream of N-ras in the control of X chromosome dosage compensation. Development 136:689-98
Abstract	Dosage compensation in Drosophila melanogaster involves the assembly of the MSL-2-containing dosage compensation complex (DCC) on the single X chromosome of male flies. Translational repression of msl-2 mRNA blocks this process in females. Previous work indicated that the ubiquitous protein Upstream of N-ras (UNR) is a necessary co-factor for msl-2 repression in vitro. Here, we explore the function of UNR in vivo. Hypomorphic Unr mutant flies showed DCC assembly on high-affinity sites in the female X chromosomes, confirming that UNR inhibits dosage compensation in female flies. Unexpectedly, male mutant flies and UNR-depleted SL2 cells showed decreased DCC binding to the X chromosome, suggesting a role for UNR in DCC assembly or targeting. Consistent with this possibility, UNR overexpression resulted in moderate loss of DCC from the male X chromosome and predominant male lethality. Immunoprecipitation experiments revealed that UNR binds to roX1 and roX2, the non-coding RNA components of the DCC, providing possible targets for UNR function in males. These results uncover dual sex-specific functions of UNR in dosage compensation: to repress DCC formation in female flies and to promote DCC assembly on the male X chromosome.
Links	PubMed Online version:10.1242/dev.027656
Keywords	Animals; Binding Sites; Cell Nucleus/metabolism; Chromatin/metabolism; DNA Transposable Elements/genetics; DNA-Binding Proteins/deficiency; DNA-Binding Proteins/metabolism; Dosage Compensation, Genetic; Drosophila Proteins/deficiency; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/genetics; Female; Fertility; Male; Protein Binding; RNA, Untranslated/metabolism; Sex Characteristics; Ubiquitin-Protein Ligases/metabolism; X Chromosome/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:B7Z0E2	enables	GO:0106222: lncRNA binding	ECO:0000314: direct assay evidence used in manual assertion		F	has_input:(FB:FBgn0019661)|has_input:(FB:FBgn0019660)	Seeded From UniProt	complete
DROME:B7Z0E2	involved_in	GO:0042714: dosage compensation complex assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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