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PMID:19168679

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Citation

Jin, H, Sood, R, Xu, J, Zhen, F, English, MA, Liu, PP and Wen, Z (2009) Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI. Development 136:647-54

Abstract

One unique feature of vertebrate definitive hematopoiesis is the ontogenic switching of hematopoietic stem cells from one anatomical compartment or niche to another. In mice, hematopoietic stem cells are believed to originate in the aorta-gonad-mesonephros (AGM), subsequently migrate to the fetal liver (FL) and finally colonize the bone marrow (BM). Yet, the differentiation potential of hematopoietic stem cells within early niches such as the AGM and FL remains incompletely defined. Here, we present in vivo analysis to delineate the differentiation potential of definitive hematopoietic stem/progenitor cells (HSPCs) in the zebrafish AGM and FL analogies, namely the ventral wall of dorsal aorta (VDA) and the posterior blood island (PBI), respectively. Cell fate mapping and analysis of zebrafish runx1(w84x) and vlad tepes (vlt(m651)) mutants revealed that HSPCs in the PBI gave rise to both erythroid and myeloid lineages. However, we surprisingly found that HSPCs in the VDA were not quiescent but were uniquely adapted to generate myeloid but not erythroid lineage cells. We further showed that such distinct differentiation output of HSPCs was, at least in part, ascribed to the different micro-environments present in these two niches. Our results highlight the importance of niche in shaping the differentiation output of developing HSPCs.

Links

PubMed PMC2646468 Online version:10.1242/dev.029637

Keywords

Animal Structures/cytology; Animal Structures/embryology; Animals; Aorta/cytology; Aorta/embryology; Cell Differentiation; Cell Lineage; Cell Movement; Embryo, Nonmammalian/cytology; Erythrocytes/cytology; Erythroid Cells/cytology; Erythropoiesis; Fertilization; Hematopoietic Stem Cells/cytology; Myeloid Cells/cytology; Zebrafish/embryology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:Q9DGB8

involved_in

GO:0060216: definitive hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-051102-3,ZFIN:ZDB-MRPHLNO-051102-4

P

Seeded From UniProt

complete

DANRE:Q9DGB8

involved_in

GO:0060216: definitive hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-091020-2

P

Seeded From UniProt

complete


See also

References

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