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PMID:19160443

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Citation

Fischer, M, Raabe, T, Heisenberg, M and Sendtner, M (2009) Drosophila RSK negatively regulates bouton number at the neuromuscular junction. Dev Neurobiol 69:212-20

Abstract

Ribosomal S6 kinases (RSKs) are growth factor-regulated serine-threonine kinases participating in the RAS-ERK signaling pathway. RSKs have been implicated in memory formation in mammals and flies. To characterize the function of RSK at the synapse level, we investigated the effect of mutations in the rsk gene on the neuromuscular junction (NMJ) in Drosophila larvae. Immunostaining revealed transgenic expressed RSK in presynaptic regions. In mutants with a full deletion or an N-terminal partial deletion of rsk, an increased bouton number was found. Restoring the wild-type rsk function in the null mutant with a genomic rescue construct reverted the synaptic phenotype, and overexpression of the rsk-cDNA in motoneurons reduced bouton numbers. Based on previous observations that RSK interacts with the Drosophila ERK homologue Rolled, genetic epistasis experiments were performed with loss- and gain-of-function mutations in Rolled. These experiments provided evidence that RSK mediates its negative effect on bouton formation at the Drosophila NMJ by inhibition of ERK signaling.

Links

PubMed Online version:10.1002/dneu.20700

Keywords

Animals; Drosophila/anatomy & histology; Drosophila/metabolism; Epistasis, Genetic; Immunohistochemistry; Mutation; Neuromuscular Junction/anatomy & histology; Neuromuscular Junction/metabolism; Presynaptic Terminals; Ribosomal Protein S6 Kinases/genetics; Ribosomal Protein S6 Kinases/metabolism; Ribosomal Protein S6 Kinases/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q24496

located_in

GO:0031594: neuromuscular junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q24496

involved_in

GO:0007528: neuromuscular junction development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q86NP0

located_in

GO:0031594: neuromuscular junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q86NP0

involved_in

GO:0007528: neuromuscular junction development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VR61

located_in

GO:0031594: neuromuscular junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VR61

involved_in

GO:0007528: neuromuscular junction development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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