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PMID:19139076
| Citation |
Liongue, C, Hall, CJ, O'Connell, BA, Crosier, P and Ward, AC (2009) Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration. Blood 113:2535-46 |
|---|---|
| Abstract |
Granulocyte colony-stimulating factor receptor (GCSFR) signaling participates in the production of neutrophilic granulocytes during normal hematopoietic development, with a particularly important role during emergency hematopoiesis. This study describes the characterization of the zebrafish gcsf and gcsfr genes, which showed broad conservation and similar regulation to their mammalian counterparts. Morpholino-mediated knockdown of gcsfr and overexpression of gcsf revealed the presence of an anterior population of myeloid cells during primitive hematopoiesis that was dependent on GCSF/GCSFR for development and migration. This contrasted with a posterior domain that was largely independent of this pathway. Definitive myelopoiesis was also partially dependent on a functional GCSF/GCSFR pathway. Injection of bacterial lipopolysaccharide elicited significant induction of gcsf expression and emergency production of myeloid cells, which was abrogated by gcsfr knockdown. Collectively, these data demonstrate GCSF/GCSFR to be a conserved signaling system for facilitating the production of multiple myeloid cell lineages in both homeostatic and emergency conditions, as well as for early myeloid cell migration, establishing a useful experimental platform for further dissection of this pathway. |
| Links |
PubMed Online version:10.1182/blood-2008-07-171967 |
| Keywords |
Amino Acid Sequence; Animals; Animals, Genetically Modified; Cell Movement/genetics; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; Hematopoiesis/genetics; Molecular Sequence Data; Myeloid Cells/metabolism; Myeloid Cells/physiology; Myeloid Progenitor Cells/metabolism; Myeloid Progenitor Cells/physiology; Myelopoiesis/genetics; Phylogeny; Receptors, Granulocyte Colony-Stimulating Factor/genetics; Receptors, Granulocyte Colony-Stimulating Factor/metabolism; Receptors, Granulocyte Colony-Stimulating Factor/physiology; Sequence Homology, Amino Acid; Signal Transduction/genetics; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
DANRE:A8Y5W0 |
involved_in |
GO:0030851: granulocyte differentiation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:A8Y5W0 |
involved_in |
GO:0030851: granulocyte differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:A8Y5W0 |
involved_in |
GO:0035162: embryonic hemopoiesis |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:A8Y5W0 |
involved_in |
GO:0030225: macrophage differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:A8Y5W0 |
involved_in |
GO:0030224: monocyte differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:A8Y5W0 |
involved_in |
GO:0030099: myeloid cell differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:A8Y5W0 |
involved_in |
GO:0060216: definitive hemopoiesis |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:B8ZHI7 |
involved_in |
GO:0035162: embryonic hemopoiesis |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:F1R668 |
involved_in |
GO:0048821: erythrocyte development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100204-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:F1R904 |
involved_in |
GO:0030851: granulocyte differentiation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:F1R904 |
involved_in |
GO:0030851: granulocyte differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:F1R904 |
involved_in |
GO:0035162: embryonic hemopoiesis |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:F1R904 |
involved_in |
GO:0030225: macrophage differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:F1R904 |
involved_in |
GO:0030224: monocyte differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:F1R904 |
involved_in |
GO:0030099: myeloid cell differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:F1R904 |
involved_in |
GO:0060216: definitive hemopoiesis |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-091229-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:Q6PHV7 |
involved_in |
GO:0048821: erythrocyte development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100204-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:Q6WEE0 |
involved_in |
GO:0048821: erythrocyte development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100204-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:Q6WEE1 |
involved_in |
GO:0048821: erythrocyte development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100204-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:Q78AM8 |
involved_in |
GO:0048821: erythrocyte development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100204-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:Q8AW41 |
involved_in |
GO:0048821: erythrocyte development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100204-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:Q8JID4 |
involved_in |
GO:0048821: erythrocyte development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-100204-2 |
P |
Seeded From UniProt |
complete | |
See also
References
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