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PMID:19139076

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Citation

Liongue, C, Hall, CJ, O'Connell, BA, Crosier, P and Ward, AC (2009) Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration. Blood 113:2535-46

Abstract

Granulocyte colony-stimulating factor receptor (GCSFR) signaling participates in the production of neutrophilic granulocytes during normal hematopoietic development, with a particularly important role during emergency hematopoiesis. This study describes the characterization of the zebrafish gcsf and gcsfr genes, which showed broad conservation and similar regulation to their mammalian counterparts. Morpholino-mediated knockdown of gcsfr and overexpression of gcsf revealed the presence of an anterior population of myeloid cells during primitive hematopoiesis that was dependent on GCSF/GCSFR for development and migration. This contrasted with a posterior domain that was largely independent of this pathway. Definitive myelopoiesis was also partially dependent on a functional GCSF/GCSFR pathway. Injection of bacterial lipopolysaccharide elicited significant induction of gcsf expression and emergency production of myeloid cells, which was abrogated by gcsfr knockdown. Collectively, these data demonstrate GCSF/GCSFR to be a conserved signaling system for facilitating the production of multiple myeloid cell lineages in both homeostatic and emergency conditions, as well as for early myeloid cell migration, establishing a useful experimental platform for further dissection of this pathway.

Links

PubMed Online version:10.1182/blood-2008-07-171967

Keywords

Amino Acid Sequence; Animals; Animals, Genetically Modified; Cell Movement/genetics; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; Hematopoiesis/genetics; Molecular Sequence Data; Myeloid Cells/metabolism; Myeloid Cells/physiology; Myeloid Progenitor Cells/metabolism; Myeloid Progenitor Cells/physiology; Myelopoiesis/genetics; Phylogeny; Receptors, Granulocyte Colony-Stimulating Factor/genetics; Receptors, Granulocyte Colony-Stimulating Factor/metabolism; Receptors, Granulocyte Colony-Stimulating Factor/physiology; Sequence Homology, Amino Acid; Signal Transduction/genetics; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A8Y5W0

involved_in

GO:0030851: granulocyte differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:A8Y5W0

involved_in

GO:0030851: granulocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:A8Y5W0

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:A8Y5W0

involved_in

GO:0030225: macrophage differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:A8Y5W0

involved_in

GO:0030224: monocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:A8Y5W0

involved_in

GO:0030099: myeloid cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:A8Y5W0

involved_in

GO:0060216: definitive hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:B8ZHI7

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:F1R668

involved_in

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100204-2

P

Seeded From UniProt

complete

DANRE:F1R904

involved_in

GO:0030851: granulocyte differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:F1R904

involved_in

GO:0030851: granulocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:F1R904

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:F1R904

involved_in

GO:0030225: macrophage differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:F1R904

involved_in

GO:0030224: monocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:F1R904

involved_in

GO:0030099: myeloid cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:F1R904

involved_in

GO:0060216: definitive hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-091229-1

P

Seeded From UniProt

complete

DANRE:Q6PHV7

involved_in

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100204-2

P

Seeded From UniProt

complete

DANRE:Q6WEE0

involved_in

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100204-2

P

Seeded From UniProt

complete

DANRE:Q6WEE1

involved_in

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100204-2

P

Seeded From UniProt

complete

DANRE:Q78AM8

involved_in

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100204-2

P

Seeded From UniProt

complete

DANRE:Q8AW41

involved_in

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100204-2

P

Seeded From UniProt

complete

DANRE:Q8JID4

involved_in

GO:0048821: erythrocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100204-2

P

Seeded From UniProt

complete


See also

References

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