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PMID:19135888

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Citation

Li, S, Makovets, S, Matsuguchi, T, Blethrow, JD, Shokat, KM and Blackburn, EH (2009) Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progression. Cell 136:50-61

Abstract

Elongation of telomeres by telomerase replenishes the loss of terminal telomeric DNA repeats during each cell cycle. In budding yeast, Cdc13 plays an essential role in telomere length homeostasis, partly through its interactions with both the telomerase complex and the competing Stn1-Ten1 complex. Previous studies in yeast have shown that telomere elongation by telomerase is cell cycle dependent, but the mechanism underlying this dependence is unclear. In S. cerevisiae, a single cyclin-dependent kinase Cdk1 (Cdc28) coordinates the serial events required for the cell division cycle, but no Cdk1 substrate has been identified among telomerase and telomere-associated factors. Here we show that Cdk1-dependent phosphorylation of Cdc13 is essential for efficient recruitment of the yeast telomerase complex to telomeres by favoring the interaction of Cdc13 with Est1 rather than the competing Stn1-Ten1 complex. These results provide a direct mechanistic link between coordination of telomere elongation and cell-cycle progression in vivo.

Links

PubMed PMC2642970 Online version:10.1016/j.cell.2008.11.027

Keywords

CDC28 Protein Kinase, S cerevisiae/metabolism; Cell Cycle; Phosphorylation; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Telomerase/metabolism; Telomere/metabolism; Telomere-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:EST1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P32797

F

Seeded From UniProt

complete

YEAST:CDC13

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17214

F

Seeded From UniProt

complete

YEAST:CDC13

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P38960

F

Seeded From UniProt

complete

YEAST:STN1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P32797

F

Seeded From UniProt

complete

YEAST:CDK1

involved_in

GO:0032210: regulation of telomere maintenance via telomerase

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P32797

P

Seeded From UniProt

complete

Notes

See also

References

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